Synergistic Effect of Newly Introduced Root Canal Medicaments; Ozonated Olive Oil and Chitosan Nanoparticles, Against Persistent Endodontic Pathogens

Frontiers in Microbiology
Mohamed I ElshinawyNehal M El-Deeb

Abstract

This study was conducted to investigate the antimicrobial-biofilm activity of chitosan (Ch-NPs), silver nanoparticles (Ag-NPs), ozonated olive oil (O3-oil) either separately or combined together against endodontic pathogens. While testing the antimicrobial activity, Ch-NPs showed the least minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values exerting eightfold higher bactericidal activity than O3-oil against both Enterococcus faecalis and Streptococcus mutans as well as fourfold higher fungicidal activity against Candida albicans. Antimicrobial synergy test revealed synergism between O3-oil and Ch-NPs against the test pathogens (FIC index ≤ 0.5). Ch-NPs was superior at inhibiting immature single and mixed-species biofilm formations by 97 and 94%, respectively. Both of O3-oil and Ch-NPs had a complete anti-fibroblast adherent effect. The safety pattern results showed that O3-oil was the safest compound, followed by Ch-NPs. The double combination of Ch-NPs and O3-oil reduced the mature viable biofilm on premolars ex vivo model by 6-log reductions, with a fast kill rate, indicating potential use in treating root canals. Therefore, the double combination has the potential to eradicate mature mi...Continue Reading

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Citations

Mar 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenjing Song, Shaohua Ge
Jan 18, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elena UgazioFranco Dosio
Dec 22, 2019·Journal of Fungi·Maria Elisa RodriguesCélia F Rodrigues
Jul 2, 2019·Science and Technology of Advanced Materials·Anongwee LeewananthawetToshihiko Suzuki
Nov 19, 2020·Nanomaterials·Hoai My TranPhong A Tran
Mar 7, 2021·Microorganisms·Katrien Van DyckPatrick Van Dijck
Jul 1, 2020·International Journal of Biological Macromolecules·Elaheh FakhriHossein Samadi Kafil
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Harinash RaoPei Pei Chong
May 19, 2021·Critical Reviews in Microbiology·Fazlurrahman KhanYoung-Mog Kim
Aug 29, 2021·Pharmaceuticals·Gustavo MoraesWalter L Siqueira

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Methods Mentioned

BETA
Assay

Software Mentioned

Graph Pad Prism
SPSS

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