Synergistic effect of silencing the expression of tick protective antigens 4D8 and Rs86 in Rhipicephalus sanguineus by RNA interference

Parasitology Research
J de la FuenteK M Kocan

Abstract

Tick proteins have been shown to be useful for the development of vaccines which reduce tick infestations. Potential tick protective antigens have been identified and characterized, in part, by use of RNA interference (RNAi). RNAi allows for analysis of gene function by characterizing the impact of loss of gene expression on tick physiology. Herein, we used RNAi in Rhipicephalus sanguineus to evaluate gene functions of two tick protective antigens, 4D8 and Rs86, the homologue of Bm86, on tick infestation, feeding and oviposition. Silencing of 4D8 alone resulted in decreased tick attachment, survival, feeding and oviposition. Although the effect of Rs86 RNAi was less pronounced, silencing of this gene also reduced tick weight and oviposition. Most notably, simultaneous silencing of 4D8 and Rs86 by RNAi resulted in a synergistic effect in which tick survival, attachment, feeding, weight and oviposition were profoundly reduced. Microscopic evaluation of tick tissues revealed that guts from dual injected ticks were distended with epithelial cells sparsely distributed along the basement membrane. These results demonstrated the synergistic effect of the silencing expression of two tick protective genes. Inclusion of multiple tick pro...Continue Reading

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Citations

Feb 23, 2010·BMC Immunology·Zorica ZivkovicJosé de la Fuente
Feb 10, 2011·Journal of Visualized Experiments : JoVE·Katherine M KocanJosé de la Fuente
Feb 20, 2014·Ticks and Tick-borne Diseases·Georg Gerhard DuscherJosé de la Fuente
Jul 7, 2012·The Veterinary Journal·Luís F PariziItabajara da Silva Vaz
Dec 2, 2008·Developmental and Comparative Immunology·Ruth C GalindoJosé de la Fuente
Mar 18, 2008·Trends in Parasitology·Peter Willadsen
Jul 28, 2007·Trends in Parasitology·José de la FuenteEdmour F Blouin
Jan 6, 2009·Annals of the New York Academy of Sciences·Edmour F BlouinKatherine M Kocan
Jan 6, 2009·Annals of the New York Academy of Sciences·José de la FuenteKatherine M Kocan
Jul 30, 2014·Parasite Immunology·E J MarrS T G Burgess
Sep 29, 2006·Trends in Parasitology·Daniel E SonenshineJosé de la Fuente
Dec 17, 2014·Archivum Immunologiae Et Therapiae Experimentalis·Girish Neelakanta, Hameeda Sultana
Jan 4, 2015·Veterinary Parasitology·José de la FuenteRuth C Galindo
Jul 12, 2019·Parasite : Journal De La Société Française De Parasitologie·Md Khalesur Rahman, Myungjo You
Jan 26, 2021·Ticks and Tick-borne Diseases·Helen Silva RibeiroRodolfo Cordeiro Giunchetti
May 1, 2021·Pathogens·Sara Artigas-JerónimoJosé de la Fuente

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