Synergistic Effect of Simple Sugars and Carboxymethyl Cellulose on the Production of a Cellulolytic Cocktail from Bacillus sp. AR03 and Enzyme Activity Characterization

Applied Biochemistry and Biotechnology
Adriana P ManfrediMaría A Martínez

Abstract

A cellulase-producing bacterium isolated from pulp and paper feedstock, Bacillus sp. AR03, was evaluated by means of a factorial design showing that peptone and carbohydrates were the main variables affecting enzyme production. Simple sugars, individually and combined with carboxymethyl cellulose (CMC), were further examined for their influence on cellulase production by strain AR03. Most of the mono and disaccharides assayed presented a synergistic effect with CMC. As a result, a peptone-based broth supplemented with 10 g/L sucrose and 10 g/L CMC maximized enzyme production after 96 h of cultivation. This medium was used to produce endoglucanases in a 1-L stirred tank reactor in batch mode at 30 °C, which reduced the fermentation period to 48 h and reaching 3.12 ± 0.02 IU/mL of enzyme activity. Bacillus sp. AR03 endoglucanases showed an optimum temperature of 60 °C and a pH of 6.0 with a wide range of pH stability. Furthermore, presence of 10 mM Mn(2+) and 5 mM Co(2+) produced an increase of enzyme activity (246.7 and 183.7 %, respectively), and remarkable tolerance to NaCl, Tween 80, and EDTA was also observed. According to our results, the properties of the cellulolytic cocktail from Bacillus sp. AR03 offer promising feature...Continue Reading

References

Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O Shoseyov, R H Doi
Feb 6, 2004·Journal of Industrial Microbiology & Biotechnology·Jagtar SinghRanbir Chander Sobti
Apr 20, 2006·Microbiology·Levente KaraffaBernhard Seiboth
Sep 15, 2009·Journal of Bacteriology·Joyce E Patrick, Daniel B Kearns
Oct 28, 2009·Journal of Industrial Microbiology & Biotechnology·Sushil NagarRamesh Chander Kuhad
Jul 6, 2010·Bioresource Technology·Gurdeep RastogiRajesh K Sani
Aug 13, 2011·Bioresource Technology·Weidong WangRuiyong Jing
Jul 27, 2012·Journal of Molecular Microbiology and Biotechnology·Miranda L MakiWensheng Qin
Apr 16, 2013·BioMed Research International·Marcella Novaes Franco-CiriglianoRosalie Reed Rodrigues Coelho
Mar 25, 2014·International Journal of Biological Macromolecules·Natesan Balasubramanian, Nelson Simões
May 2, 2014·Frontiers in Microbiology·Rajeshwari Sinha, Sunil K Khare
Jan 1, 2013·Genomics Insights·Archana Sharma, T Satyanarayana
Aug 20, 2015·Applied Biochemistry and Biotechnology·Yagya Prasad Paudel, Wensheng Qin
Sep 16, 2015·Journal of Basic Microbiology·Adriana P ManfrediMaría A Martínez

❮ Previous
Next ❯

Citations

Jan 21, 2017·Preparative Biochemistry & Biotechnology·Johan S HeroMaría A Martínez
Nov 23, 2017·Journal of Molecular Microbiology and Biotechnology·José H PisaMaría Alejandra Martínez
Sep 14, 2019·Preparative Biochemistry & Biotechnology·Isabela da Silva Vasconcelos RodriguesRoberta Pereira Miranda Fernandes
Jul 1, 2017·Environmental Science and Pollution Research International·Enzo Di MarcoMaría Alejandra Martínez

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.