Synergistic effect of testosterone and of a luteinizing hormone-releasing hormone agonist on androgen receptor content in the ventral prostate of castrated rats

Steroids
G FiorelliM Motta

Abstract

The aim of the present experiment was that of studying the effect of an LHRH agonist analog on the prostatic content of cytosol and nuclear salt-extractable and salt-resistant androgen receptors (AR). Castrated rats were treated for six days with the LHRH agonist WY 40972 (A), with testosterone enanthate (T) or with A plus T. Intact adult male rats and castrated rats treated with the vehicle served as controls. The animals were sacrificed 18 h after the last subcutaneous injection. The ventral prostates were quickly removed and submitted to subcellular fractionation for the determination of cytosol and nuclear AR content. In addition, the weights of the prostates and of the seminal vesicles were recorded, and serum levels of LH and FSH were evaluated by radioimmunoassay. The dissociation constants (Kd) of cytosol and nuclear AR, on the order of 1 x 10(-9) M, were not affected by the various treatments. Conversely, the combined treatment with T and A induced a significant increase of nuclear AR in the prostatic tissue, when compared to the levels found in castrated rats treated with T alone and in intact rats. The treatment with T was able to restore the reproductive organs to their normal weights. The treatment with A inhibited...Continue Reading

References

Jul 15, 1978·The Biochemical Journal·E Van Doorn, N Bruchovsky
Jul 1, 1986·The Journal of Clinical Endocrinology and Metabolism·S ZoppiM Motta
Jul 1, 1968·Proceedings of the Society for Experimental Biology and Medicine·G D NiswenderL E Reichert
Sep 1, 1980·Analytical Biochemistry·P J Munson, D Rodbard
Jan 1, 1981·Endocrine Reviews·A J Hsueh, P B Jones
Apr 5, 1983·FEBS Letters·M T HierowskiA V Schally
Nov 1, 1984·Journal of Reproduction and Fertility·K SundaramC W Bardin
Nov 1, 1980·Molecular and Cellular Endocrinology·F A LefebvreF Labrie

❮ Previous
Next ❯

Citations

Feb 1, 1995·Calcified Tissue International·M PetilliM L Brandi
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·J SandowG Jerabek-Sandow
Jan 1, 1991·The Journal of Steroid Biochemistry and Molecular Biology·L J BlockJ A Grootegoed
Mar 1, 1996·The Journal of Steroid Biochemistry and Molecular Biology·R ShenN F Gonzalez-Cadavid
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·G FiorelliM L Brandi
Jul 1, 1997·International Journal of Urology : Official Journal of the Japanese Urological Association·H MaezawaA Ueno

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.