Abstract
Due to their direct antiviral activity, Mx proteins play a main role in the response mediated by type I interferon against viral infections. The study on gilthead seabream Mx proteins is especially interesting, since this species is unusually resistant to viral diseases, being asymptomatic carrier of several viruses pathogenic to other fish species. Gilthead seabream has three Mx proteins (Mx1, Mx2 and Mx3) that, separately, display antiviral activity against a wide range of viruses, showing interesting differences in their antiviral specificities. In this work, the possible synergy between the three Mx isoforms has been studied using in vitro systems consisting of CHSE-214 cells stably expressing two or the three gilthead seabream Mx proteins. The antiviral activity of these Mx combinations has been tested against the Infectious Pancreatic Necrosis Virus (IPNV), the Viral Haemorrhagic Septicaemia Virus (VHSV), the European Sheatfish Virus (ESV) and the Lymphocystis Disease Virus (LCDV). A synergistic effect of the Mx proteins was only detected against ESV, no synergy was observed against LCDV, and a negative interference was detected against the two RNA viruses tested, IPNV and VHSV, as viral replication was higher in cells ex...Continue Reading
References
Feb 25, 1966·Science·K WolfR G Malsberger
Aug 10, 1965·Annals of the New York Academy of Sciences·J L FryerK S Pilcher
Dec 16, 1983·Journal of Immunological Methods·T Mosmann
Jun 25, 1998·Revue Scientifique Et Technique·O HallerG Kochs
Jun 26, 2001·Veterinary Microbiology·S Perez-PrietoJ J Borrego
Oct 5, 2001·Clinical Microbiology Reviews·C E Samuel
Jan 19, 2002·Diseases of Aquatic Organisms·J CastriJ C Raymond
Jul 16, 2004·Journal of Virology·Rannveig LarsenBørre Robertsen
Jan 18, 2006·Methods in Enzymology·Georg KochsOtto Haller
Dec 13, 2006·Fish & Shellfish Immunology·C TafallaA Estepa
Jun 16, 2007·Biochimie·Otto HallerGeorg Kochs
Jul 29, 2008·Fish & Shellfish Immunology·Maria Angeles EstebanAlberto Cuesta
Oct 25, 2008·Fish & Shellfish Immunology·Kosuke Zenke, Ki Hong Kim
Apr 17, 2009·Molecular Biology and Evolution·Guang LiYiquan Wang
May 26, 2009·Virus Research·Astrid SkjesolJorunn B Jørgensen
May 22, 2010·Journal of Fish Diseases·I CanoD Castro
Dec 21, 2010·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Otto Haller, Georg Kochs
Apr 7, 2011·Molecular Immunology·M A Fernández-TrujilloJ Béjar
Apr 12, 2011·Nature·John W SchogginsCharles M Rice
Aug 30, 2011·Molecular Immunology·M A Fernández-TrujilloJ Béjar
Oct 4, 2011·Immunity·Song GaoOliver Daumke
Jan 10, 2012·Fish & Shellfish Immunology·Min Sun Kim, Ki Hong Kim
Mar 13, 2012·Journal of Virological Methods·Katherine LesterBertrand Collet
Aug 14, 2012·Marine Biotechnology·Daniel Alvarez-TorresM Carmen Alonso
Oct 23, 2012·Cell Host & Microbe·Patrick S MitchellGeorg Kochs
Apr 4, 2013·Veterinary Immunology and Immunopathology·P NovelJ Béjar
Aug 6, 2013·Molecular Immunology·M A Fernández-TrujilloJ Béjar
Nov 29, 2013·Nature·John W SchogginsCharles M Rice
Apr 8, 2014·Fish & Shellfish Immunology·J A González-MariscalJ Béjar