PMID: 15388887Sep 25, 2004Paper

Synergistic effects of ANP and sildenafil on cGMP levels and amelioration of acute hypoxic pulmonary hypertension

Experimental Biology and Medicine
Ioana R PrestonJ R Klinger

Abstract

We hypothesized that the phosphodiesterase 5 inhibitor, sildenafil, and the guanosine cyclase stimulator, atrial natriuretic peptide (ANP), would act synergistically to increase cGMP levels and blunt hypoxic pulmonary hypertension in rats, because these compounds act via different mechanisms to increase the intracellular second messenger. Acute hypoxia: Adult Sprague-Dawley rats were gavaged with sildenafil (1 mg/ kg) or vehicle and exposed to acute hypoxia with and without ANP (10(-8)-10(-5) M ). Sildenafil decreased systemic blood pressure (103 +/- 10 vs. 87 +/- 6 mm Hg, P < 0.001) and blunted the hypoxia-induced increase in right ventricular systolic pressure (RVSP; percent increase 73.7% +/- 9.4% in sildenafil-treated rats vs. 117.2% +/- 21.1% in vehicle-treated rats, P = 0.03). Also, ANP and sildenafil had synergistic effects on blunting the hypoxia-induced increase in RVSP (P < 0.001) and on rising plasma cGMP levels (P < 0.05). Chronic hypoxia: Other rats were exposed to prolonged hypoxia (3 weeks, 0.5 atm) after subcutaneous implantation of a sustained-release pellet containing lower (2.5 mg), or higher (25 mg) doses of sildenafil, or placebo. Higher-dose, but not lower-dose sildenafil blunted the chronic hypoxia-induce...Continue Reading

References

Jan 1, 1990·Pulmonary Pharmacology·N A NumanR J Altiere
Feb 1, 1991·The American Journal of Physiology·R D Specian, M G Oliver
Nov 1, 1981·Experimental Lung Research·B Meyrick, L Reid
Aug 2, 1994·Proceedings of the National Academy of Sciences of the United States of America·S L ArcherE K Weir
Jul 1, 1993·Journal of Applied Physiology·J R KlingerN S Hill
Jun 23, 1998·The Journal of Biological Chemistry·S H SoderlingJ A Beavo
Apr 29, 1999·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·D K WalkerP A Wright
Nov 11, 1999·International Journal of Impotence Research·C M GonzalezK T McVary
Jul 27, 2001·Circulation·L ZhaoM R Wilkins
Aug 30, 2003·Circulation Research·Vincent SauzeauPierre Pacaud

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Citations

Aug 8, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Mark KirschHarald H H W Schmidt
Jun 28, 2011·Expert Review of Respiratory Medicine·James R Klinger
May 31, 2018·British Journal of Pharmacology·Tara Elizabeth ScottAdrian J Hobbs
Jun 17, 2006·Journal of Applied Physiology·Pierre FeslerRobert Naeije
Aug 24, 2006·Expert Opinion on Pharmacotherapy·Sabina A Antoniu
May 22, 2007·Antioxidants & Redox Signaling·Ioana R Preston
Nov 22, 2017·European Journal of Applied Physiology·Thomas MondritzkiHubert Truebel
May 26, 2010·Korean journal of anesthesiology·Ji-Yeon Sim
Dec 24, 2010·British Journal of Pharmacology·Reshma S BaligaAdrian J Hobbs
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Suhayla Mukaddam-Daher
Aug 8, 2009·International Journal of Experimental Pathology·Karina Lidianne Alcântara SaraivaChristina Alves Peixoto
Aug 12, 2008·American Journal of Respiratory and Critical Care Medicine·Reshma S BaligaAdrian J Hobbs
Mar 5, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ioana R PrestonIris Z Jaffe
May 2, 2015·The European Respiratory Journal·David Domínguez-FandosJoan A Barberà
Feb 14, 2021·International Journal of Environmental Research and Public Health·Akylbek SydykovRalph Theo Schermuly
Jul 3, 2021·Pulmonary Circulation·Divya P MenonIoana R Preston
Nov 21, 2007·European Journal of Cell Biology·Gopi Krishna KolluruSuvro Chatterjee

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