Synergistic effects of beta-aescin and 5-fluorouracil in human hepatocellular carcinoma SMMC-7721 cells

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
Z J MingX G Zhang

Abstract

The effects and mechanisms of action of beta-aescin and 5-fluorouracil (5-FU), alone and in combination, were studied in human hepatocellular carcinoma SMMC-7721 cells. Growth inhibition, cell cycle distribution, apoptosis, Bcl-2 expression and caspase activity were assessed. The Isobole-method/interaction-index analysis was applied to evaluate the synergy, additivity or antagonism of these agents. The results indicate that mixtures of beta-aescin and 5-FU showed a synergistic effect on the 50% inhibitory effect when their ratio was 4:1 when compared with either agent alone. The mechanism of action could be through the synergistic arrest of the cell cycle, induction of apoptosis, activation of caspases-3, 8 and 9, and down-regulation Bcl-2 expression. The results suggest that mixtures of these two agents had a synergistic inhibitory effect on SMMC-7721 cells, an observation which might be useful for the further development of anti-cancer drugs.

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Citations

Dec 14, 2011·Chinese Journal of Integrative Medicine·Xue-Lin WangXiong-Zhi Wu
Jul 3, 2013·Cancer Biotherapy & Radiopharmaceuticals·Rongrong ZhuShilong Wang
Dec 20, 2011·International Journal of Oncology·Kazuya UchiboriNobunori Sato
Jun 22, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hui WangRui Ling
Aug 23, 2012·Phytotherapy Research : PTR·G GüneyA Işcan
May 23, 2013·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Huanli XuGuanhua Du
May 21, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Hai-ya HuangYan-hua Lu
Jan 7, 2015·Critical Reviews in Analytical Chemistry·Monika WieczerzakJacek Namieśnik
Dec 22, 2012·International Archives of Allergy and Immunology·Wolfgang SiposPetra Doerfler
Feb 6, 2020·Pharmaceutical Development and Technology·Xiaolin Fang, Aizong Shen
Jul 2, 2020·Seminars in Cancer Biology·Elina KhatoonAjaikumar B Kunnumakkara
Dec 31, 2020·Plants·Nourhan Hisham ShadyUsama Ramadan Abdelmohsen
Apr 20, 2019·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Sonali GhoshManju Ray

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