Synergistic effects of Buyang Huanwu decoction and embryonic neural stem cell transplantation on the recovery of neurological function in a rat model of spinal cord injury

Experimental and Therapeutic Medicine
Min ZhangCheng Yang

Abstract

The aim of the present study was to investigate the therapeutic effect of a combined treatment of Buyang Huanwu decoction (BYHWD), a well-known formula of traditional Chinese medicine, and neural stem cells (NSCs) on spinal cord injury (SCI) and the associated underlying mechanisms. A SCI model was established by surgery via a complete transection of the T10 vertebra of female Sprague-Dawley rats. Gelatin sponges were used to absorb NSCs labeled with the thymidine analog, 5-bromo-2-deoxyuridine (BrdU), and were transferred into the transected spinal cords. BYHWD was administered once a day by introgastric infusion. Motor functions of the hind limbs were evaluated using the 21-point locomotor rating scale developed by Basso, Beattie and Bresnahan (BBB). The fate of the transplanted NSCs under the various conditions was examined by double immunofluorescence staining, using markers for neurons, astrocytes and oligodendrocytes, with BrdU. Ultrastructural changes of the SCI site following the various treatments were examined under a transmission electron microscope. The number of double positive cells for glial fibrillary acidic protein and BrdU in the BYHWD + NSC group was significantly decreased when compared with that in the NSC ...Continue Reading

References

Feb 1, 1995·Journal of Neurotrauma·D M BassoJ C Bresnahan
May 20, 1999·Journal of Neuropathology and Experimental Neurology·J Namiki, C H Tator
May 18, 2000·Nature Neuroscience·A Björklund, O Lindvall
Jan 16, 2002·The American Journal of Chinese Medicine·Y S ChengC C Tsai
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yang D TengEvan Y Snyder
Aug 20, 2003·The European Journal of Neuroscience·Maurice VroemenNorbert Weidner
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·Paul J Reier
Aug 10, 2005·PLoS Biology·Luciano ContiAustin Smith
Sep 21, 2005·Proceedings of the National Academy of Sciences of the United States of America·Brian J CummingsAileen J Anderson
Jan 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Donghui YangYuying Zhao
Mar 31, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Soheila Karimi-AbdolrezaeeMichael G Fehlings
Aug 26, 2006·Stem Cells·Yao Fei HuMaya Sieber-Blum
Jul 6, 2010·Brain Research·Suelen Adriani MarquesAna Maria Blanco Martinez
Mar 29, 2014·Nature·Eunju KangShoukhrat Mitalipov

❮ Previous
Next ❯

Citations

Apr 27, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yubao LuXuegong Fan
May 13, 2021·Evidence-based Complementary and Alternative Medicine : ECAM·Zhencheng XiongPing Yi

❮ Previous
Next ❯

Software Mentioned

Pro Plus
SPSS
Image

Related Concepts

Related Feeds

Blood Brain Barrier Chips

The blood brain barrier (BBB) is comprised of endothelial cells that regulate the influx and outflux of plasma concentrations. Lab-on-a-chip devices allow scientists to model diseases and mechanisms such as the passage of therapeutic antibodies across the BBB. Discover the latest research on BBB chips here.

Blood Brain Barrier Regulation in Health & Disease

The blood brain barrier is essential in regulating the movement of molecules and substances in and out of the brain. Disruption to the blood brain barrier and changes in permeability allow pathogens and inflammatory molecules to cross the barrier and may play a part in the pathogenesis of neurodegenerative disorders. Here is the latest research in this field.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

Blood Brain Barrier

The blood brain barrier is a border that separates blood from cerebrospinal fluid. Discover the latest search on this highly selective semipermeable membrane here.