PMID: 11920661Mar 29, 2002Paper

Synergistic effects of glucose and ultraviolet irradiation on the physical properties of collagen

Journal of Biomedical Materials Research
Mark P OhanMichael G Dunn

Abstract

Our objective was to strengthen and stabilize collagen films without the introduction of cytotoxic chemical crosslinkers. We hypothesized that collagen could be rapidly crosslinked with glucose with ultraviolet (UV) irradiation as a catalyst. In theory, UV-generated free radicals can expedite collagen crosslinking with glucose via the formation of reactive, linear glucose molecules. The mechanical properties of glucose-incorporated, UV-exposed collagen films and appropriate controls were determined under various conditions: (1) hydration in phosphate-buffered saline, (2) heat-denaturation, (3) incubation in a collagenase solution, and (4) incubation in a trypsin solution. Without exposure to UV, the incorporation of glucose into the films had no effect. Without glucose, exposure to UV increased the strength but caused significant denaturation. The combination of glucose and UV, however, synergistically improved the mechanical properties and enzyme resistance of collagen films, indicative of increased crosslinking without significant denaturation effects. The addition of thiourea, a potent free-radical scavenger, or aminoguanidine, an inhibitor of glucose-derived crosslinking, to the collagen films markedly hindered these synerg...Continue Reading

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Citations

Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Ronald DorotkaStefan Nehrer
Jun 5, 2004·International Journal of Biological Macromolecules·Alina Sionkowska, Tim Wess
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