PMID: 20657156Jul 27, 2010Paper

Synergistic effects of glutathione and β-mercaptoethanol treatment during in vitro maturation of porcine oocytes on early embryonic development in a culture system supplemented with L-cysteine

The Journal of Reproduction and Development
Changyong ChoeDawon Kang

Abstract

Various methods have been used to remove reactive oxygen species (ROS) generated from in vitro culture (IVC) conditions that can cause cell injury or death, including the application of low oxygen (O(2)) tension and the addition of antioxidants. The beneficial effects of antioxidants and O(2) tension on IVC of porcine embryos, however, are controversial among researchers. In this study, we sought to determine the effects and optimal concentrations of antioxidants for the development of porcine embryos in an IVC system. Specifically, we examined the synergistic effects of antioxidants on development to the blastocyst stage in a culture system supplemented with L-cysteine during IVM. Of the antioxidants tested (melatonin, glutathione (GSH), β-mercaptoethanol (β-ME), N-acetylcysteine (NAC) and dithiothreitol (DTT)), addition of GSH (1 mM) or β-ME (25 µM) significantly increased development to the blastocyst stage compared with the controls without antioxidant treatment (22.2 ± 4.2% for 1 mM GSH, 25.9 ± 2.2% for 25 µM β-ME and 12-13% for the control, P<0.05). In addition, the mean cell number per blastocyst was increased by approximately 1.7-fold in the presence of GSH or β -ME. These GSH- and β-ME-induced increases in development ...Continue Reading

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Citations

Sep 18, 2013·Reproduction : the Official Journal of the Society for the Study of Fertility·Hua-Yu LianJing-He Tan
Apr 1, 2014·Journal of Assisted Reproduction and Genetics·V A Absalón-MedinaR O Gilbert
Mar 28, 2012·The Journal of Reproduction and Development·Masashi Takahashi
Aug 5, 2014·The Journal of Reproduction and Development·Robert E Click
Jun 16, 2012·Animal Reproduction Science·Wei ZhangXu Zhou
Nov 14, 2013·Oxidative Medicine and Cellular Longevity·Elisa GiarettaGiovanna Galeati
May 12, 2016·Human Reproduction·Thi T TruongDavid K Gardner
Jan 30, 2015·Journal of Assisted Reproduction and Genetics·Zohreh ZareMaryam Dehghani-Mohammadabadi

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