PMID: 6974753Jan 1, 1981Paper

Synergistic effects of human B and T lymphocyte mitogens induce uniform, high levels of immunoglobulin secretion among normal donors

Journal of Immunological Methods
O Saiki, P Ralph

Abstract

Synergistic effects of B-cell mitogen Staphylococcus bacteria strain Cowan I (Cowan I) plus T-cell activator pokeweed mitogen (PWM) in generating immunoglobulin-secreting cells (ISC) from human peripheral blood mononuclear cells (MNC) were investigated. ISC were assayed by reverse plaque-forming cells uing protein A-coated red blood cells. Low concentrations of PWM plus Cowan I gave superadditive effects on ISI induction, generating 3-20 times as many ISC as optimal amounts of either mitogen alone. The mitogens together and separately showed similar kinetics of ISC; synergy was observed at every day tested. IgM ISC represented 10% of initial MNC from all cell donors tested even if the donors were low responders to either mitogen alone. The numbers of ISC obtained are higher than previous reports and more uniform among donors, making this a superlative method for studies on normal human immunoglobulin secretion.

References

Feb 1, 1978·European Journal of Immunology· LêthibichthuyJ Brochier
Jul 1, 1978·The Journal of Experimental Medicine·C BonaR G Miller
Mar 1, 1976·European Journal of Immunology·A ForsgrenH Wigzell
Sep 1, 1980·Cellular Immunology·J T KurnickH Wigzell

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Citations

Mar 1, 1987·Veterinary Immunology and Immunopathology·R J ScibienskiJ L Stott
Sep 1, 1984·Journal of Clinical Immunology·A MuraguchiA S Fauci

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