Synergistic Effects of Melittin and Plasma Treatment: A Promising Approach for Cancer Therapy

Cancers
Priyanka ShawAnnemie Bogaerts

Abstract

Melittin (MEL), a small peptide component of bee venom, has been reported to exhibit anti-cancer effects in vitro and in vivo. However, its clinical applicability is disputed because of its non-specific cytotoxicity and haemolytic activity in high treatment doses. Plasma-treated phosphate buffered saline solution (PT-PBS), a solution rich in reactive oxygen and nitrogen species (RONS) can disrupt the cell membrane integrity and induce cancer cell death through oxidative stress-mediated pathways. Thus, PT-PBS could be used in combination with MEL to facilitate its access into cancer cells and to reduce the required therapeutic dose. The aim of our study is to determine the reduction of the effective dose of MEL required to eliminate cancer cells by its combination with PT-PBS. For this purpose, we have optimised the MEL threshold concentration and tested the combined treatment of MEL and PT-PBS on A375 melanoma and MCF7 breast cancer cells, using in vitro, in ovo and in silico approaches. We investigated the cytotoxic effect of MEL and PT-PBS alone and in combination to reveal their synergistic cytological effects. To support the in vitro and in ovo experiments, we showed by computer simulations that plasma-induced oxidation of ...Continue Reading

References

Aug 1, 1991·Archives of Biochemistry and Biophysics·R RadiB A Freeman
Mar 1, 1985·Physical Review A: General Physics·W G Hoover
Aug 18, 2001·Biophysical Journal·L YangH W Huang
Jul 29, 2004·Current Pharmaceutical Design·Carola Leuschner, William Hansel
Feb 8, 2005·Journal of the American Society for Mass Spectrometry·Jason W H WongKevin M Downard
Oct 8, 2005·Journal of Computational Chemistry·David Van Der SpoelHerman J C Berendsen
Nov 15, 2006·Molecular and Cellular Endocrinology·William HanselCarola Leuschner
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sudha Dorairaj, Toby W Allen
Sep 4, 2007·Biophysical Journal·Jirasak Wong-EkkabutLuca Monticelli
Oct 16, 2007·Toxicon : Official Journal of the International Society on Toxinology·Dong-Oh MoonGi-Young Kim
Dec 11, 2007·Biochemical Pharmacology·Sam Maher, Siobhán McClean
Jul 8, 2008·Toxicon : Official Journal of the International Society on Toxinology·Wu-Chun TuShih-Lan Hsu
Feb 21, 2009·Journal of Computational Chemistry·L MartínezJ M Martínez
Jun 10, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Wael SolimanKamaljit Kaur
Aug 27, 2009·The Journal of Physical Chemistry. B·See-Wing ChiuEric Jakobsson
Oct 1, 2009·Arhiv za higijenu rada i toksikologiju·Nada Orsolić
Jan 13, 2011·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Hua PanSamuel A Wickline
Jan 25, 2011·Nature Reviews. Cancer·Rob A CairnsTak W Mak
Mar 2, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Moutusi Manna, Chaitali Mukhopadhyay
Aug 11, 2012·Biochimica Et Biophysica Acta·Sheeba J Irudayam, Max L Berkowitz
Mar 27, 2013·Biophysical Journal·Magnus AnderssonStephen H White
Jul 31, 2013·Environmental Toxicology and Pharmacology·Goran Gajski, Vera Garaj-Vrhovac
Oct 15, 2013·The Journal of Physical Chemistry. B·Sheeba J IrudayamMax L Berkowitz
Mar 4, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Cheng BeiXu Peisheng
Dec 15, 2015·Trends in Cell Biology·Wan Seok Yang, Brent R Stockwell
Jan 21, 2016·Archives of Medical Science : AMS·Shiva IraniShahriar Mirpoor
Apr 6, 2017·Scientific Reports·Oleg LunovŠárka Kubinová
Jul 18, 2017·Molecular Medicine Reports·Su-Fang Zhang, Zhe Chen

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Citations

Sep 10, 2020·Antibodies·Ronald P Taylor, Margaret A Lindorfer
Jul 28, 2020·Cancers·Gabriella Pasqual-MeloSander Bekeschus
Aug 25, 2020·Plasma Processes and Polymers·Dawei LiuKostya Ken Ostrikov
Dec 5, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Sepideh MirzaeiManoj Garg
Feb 27, 2021·Free Radical Biology & Medicine·Naresh KumarAnnemie Bogaerts
Mar 7, 2021·Journal of Clinical Medicine·Miguel Mateu-SanzCristina Canal
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Helena MotalnBoris Rogelj
Jul 3, 2021·Cancers·Gabrielle WishartGiuseppe Schettino

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Methods Mentioned

BETA
MDA
Assay
flow cytometry
flow
fluorescence microscopy

Software Mentioned

GROMACS
Packmol
GROMOS
Prism
Excel

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