Synergistic effects of polymyxin and ionic liquids on lipid vesicle membrane stability and aggregation

Biophysical Chemistry
Sylvia L HannaTimothy D Vaden

Abstract

Ionic liquids (ILs) have been investigated for potential antibacterial and antibiotic applications due to their ability to destabilize and permeabilize the lipid bilayers in cell membranes. Bacterial assays have shown that combining ILs with antibiotics can provide a synergistic enhancement of their antibacterial activities. We have characterized the mechanism by which the conventional ILs 1-butyl-3-methylimidazolium chloride (BMICl) and 1-butyl-3-methylimidazolium tetrafluoroborate (BMIBF4) enhance the lipid membrane permeabilization of the well-known antibiotic polymyxin B (PMB). We studied the sizes and membrane permeabilities of multilamellar and unilamellar lipid bilayer vesicles in the presence of ILs alone in aqueous solution, PMB alone, and ILs combined together with PMB. Light scattering-based experiments show that vesicle sizes dramatically increase when ILs are combined with PMB, which suggests that the materials combine to synergistically enhance lipid membrane disruption leading to vesicle aggregation. Lipid bilayer leakage experiments using tris (2,2'-bipyridyl) ruthenium (II) (Ru(bpy)3(2+)) trapped in lipid vesicles, in which the trapped Ru(bpy)3(2+) fluorescence lifetime increases when it leaks out of the vesicl...Continue Reading

Citations

Nov 6, 2017·Probiotics and Antimicrobial Proteins·Kimberly D Saint JeanGregory A Caputo
Sep 6, 2020·Probiotics and Antimicrobial Proteins·Morgan A HitchnerGregory A Caputo
Dec 29, 2020·Nanomedicine·Mariana AmaralCatarina Pinto Reis
Feb 8, 2021·Drug Discovery Today·Manuel SimõesFernanda Borges
Jul 25, 2019·The Journal of Physical Chemistry. B·Arusha AcharyyaTimothy D Vaden
May 11, 2019·Biochimica Et Biophysica Acta. Biomembranes·Morgan A HitchnerGregory A Caputo

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