Synergistic effects of tea polyphenol epigallocatechin 3-O-gallate and azole drugs against oral Candida isolates

Journal de mycologie médicale
Jawad BehbehaniMaribassapa Karched

Abstract

The antifungal drug resistance has become an emerging problem in the management of candida infections worldwide. The objective of this study was to examine the efficacy of epigallocatechin 3-O-gallate (EGCG) alone and in combination with fluconazole/ketoconazole drugs against oral Candida isolates. Minimum inhibitory concentration (MIC) and minimum fungicidal concentrations (MFC) of EGCG against 60 oral Candida isolates and 4 ATCC strains were determined. Synergism of EGCG with azole drugs was evaluated by checkerboard micro-dilution method and calculated fractional inhibitory concentration index (FICI). Candida cells' ultrastructure was studied by electron microscopy. MIC and MFC values of EGCG were in the range of 3.91-15.63 and 15.63-31.25μg/mL, respectively. Minimum biofilm inhibitory concentration (MBIC) range of EGCG (62.5-125μg/mL), was less than the ketoconazole (64-256μg/mL) and fluconazole (128-512μg/mL). The combination of EGCG with fluconazole/ketoconazole exhibited synergistic effects (ΣFICI≤0.50). EGCG with azole drugs showed high sensitivity against the tested isolates in growth curve assays. Against the biofilm, the susceptibility of fluconazole/ketoconazole significantly increased (3 to 5 fold), after combinati...Continue Reading

Citations

Jun 23, 2021·Journal of Endodontics·Andiara De RossiLea Assed Bezerra da Silva

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