Synergistic induction of polypeptides by tumor necrosis factor and interferon-gamma in cells sensitive or resistant to tumor necrosis factor: assessment by computer based analysis of two-dimensional gels using the PDQUEST system

Electrophoresis
M H BeresiniL B Epstein

Abstract

Tumor necrosis factor (TNF) synergistically enhanced the antiproliferative activity of interferon-gamma (IFN-gamma) in both TNF-sensitive and TNF-resistant variants of the cervical carcinoma line, ME-180. TNF alone had no apparent effect on the levels of synthesis of individual proteins in either of these variant cell lines as assessed by computerized two-dimensional gel analysis of cell lysates using the PDQUEST system. However, IFN-gamma enhanced the levels of 18 polypeptides and suppressed the levels of 10 polypeptides in both cell lines. When used in combination in both cell lines, TNF and IFN-gamma induced the synthesis of 10 polypeptides that were not induced by either agent alone. These synergistically induced polypeptides may be crucial to the mechanism of the synergistic antiproliferative action of TNF and IFN-gamma in ME-180 cells.

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Citations

Dec 15, 2007·Veterinary Research Communications·Chuang Xu, Zhe Wang
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S C HallA L Burlingame
Dec 1, 1994·Journal of Interferon Research·S B KleinM W Taylor
Jan 1, 1996·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·I RaineriL B Epstein
Jan 1, 1995·American Journal of Human Biology : the Official Journal of the Human Biology Council·E J ParraJ L B Caeiro
Sep 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Niki L ReynaertYvonne M W Janssen-Heininger
Oct 26, 2010·Journal of Proteome Research·Leonardo MurgianoLello Zolla

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