Synergistic interaction caused to human gingival fibroblasts from dental monomers

Dental Materials : Official Publication of the Academy of Dental Materials
Jürgen DurnerF X Reichl

Abstract

The toxicity of monomers like bisphenol-A-glycidyldimethacrylate (BisGMA) and urethane-dimethacrylate (UDMA) to cells is well studied. In these studies solubilizers, which have a toxic potential, are used to dissolve the basic monomers in the aqueous medium. In these experiments it is not possible to confidently exclude a synergistic effect of basic monomers and solubilizers in cells. Moreover, less is known about the synergistic interaction between basic- and comonomers (triethyleneglycoldimethacrylate (TEGDMA); 2-hydroxyethylmethacrylate (HEMA)) in cells. We dissolved the basic monomers in the comonomers and incubated human gingival fibroblasts (HGFs) with these binary mixtures in different concentrations. Proliferating HGFs monolayers were cultured in the absence or presence of mixtures of BisGMA/TEGDMA, BisGMA/HEMA, UDMA/TEGDMA and UDMA/HEMA. Twenty-four hours later XTT was added and the formazan formation was quantified. EC(50) values were obtained at half-maximum-effect concentrations from fitted curves. EC(50) values were (mmol/l; mean±sem; n=5): 0.01 BisGMA/0.48 TEGDMA; 0.04 BisGMA/4.99 HEMA; 0.04 UDMA/1.60 TEGDMA and 0.02 UDMA/2.26 HEMA. All tested mixtures induced a dose-dependent loss of viability in HGFs after 24h. ...Continue Reading

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Mar 30, 2011·Dental Materials : Official Publication of the Academy of Dental Materials·Jürgen DurnerFranx-Xaver Reichl

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Citations

Aug 24, 2013·Acta Odontologica Scandinavica·Antonija TadinDavor Zeljezic
Apr 11, 2013·Dental Materials : Official Publication of the Academy of Dental Materials·S FurcheJ Durner
Dec 18, 2013·Journal of Biomedical Materials Research. Part a·Marialucia GalloriniViviana di Giacomo
Apr 25, 2013·Journal of Biomedical Materials Research. Part a·V AnsteinssonJ T Samuelsen
Oct 12, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Stevan M CokicKirsten L Van Landuyt
May 17, 2018·Oral Diseases·Carlo BertoldiEmanuela Monari
Dec 12, 2017·Dental Materials Journal·Aysegul KurtAslihan Usumez
Nov 13, 2019·Clinical Oral Investigations·Susana BandarraIsabel Barahona
Apr 15, 2021·Journal of Orofacial Orthopedics = Fortschritte Der Kieferorthopädie : Organ/official Journal Deutsche Gesellschaft Für Kieferorthopädie·B J KuxP-G Jost-Brinkmann
Aug 30, 2021·Dental Materials : Official Publication of the Academy of Dental Materials·Jürgen DurnerMiriam E Draenert

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