Synergistic interaction of interleukin-1 beta and growth factors in primary cultures of rat aortic smooth muscle cells

Journal of Cellular Physiology
T BourcierA Hassid

Abstract

Activated macrophages release cytokines and growth factors that may contribute to the growth of vascular smooth muscle cells in injured blood vessels. In the present study, we investigated the interactions between interleukin-1 beta (IL-1 beta) and basic fibroblast growth factor (FGF-2) in primary rat aortic smooth muscle cells, relative to their effects on DNA synthesis and cell proliferation. We report that femtomolar levels of IL-1 beta, which alone were non-mitogenic or weakly mitogenic, synergistically increased FGF-2-induced [3H]thymidine incorporation and cell proliferation. The potentiating effect of IL-1 beta extended to PDGF-AB and EGF, but not to IGF-1-induced thymidine incorporation. An antagonist of the IL-1 receptor, IL-1ra, blocked the co-mitogenic effect of IL-1 beta. Stimulation of cells with FGF-2 and IL-1 beta increased both DNA content and proliferation, an observation that was consistent with the thymidine incorporation experiments. An inhibitor of NO synthase, N5-iminoethyl L-ornithine (L-NIO), did not block the co-mitogenic effect of IL-1 beta, despite effective inhibition of NO synthase activity, suggesting that the synergistic interaction between IL-1 beta and FGF-2 was independent of the NO/cGMP pathwa...Continue Reading

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Citations

Mar 29, 2001·Journal of Hematotherapy & Stem Cell Research·L HegyiJ N Skepper
Dec 14, 2011·Clinical Lipidology·Mei-Zhen Cui
Feb 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Nan ChenJeng-Jiann Chiu
Jan 15, 2013·The American Journal of Pathology·Jean-Philippe GiotLaure Favot
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Sep 28, 1998·General and Comparative Endocrinology·T ShimadaH Nishimura

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