PMID: 8970984Dec 1, 1996Paper

Synergistic interactions between overlapping binding sites for the serum response factor and ELK-1 proteins mediate both basal enhancement and phorbol ester responsiveness of primate cytomegalovirus major immediate-early promoters in monocyte and T-lymphocyte cell types

Journal of Virology
Y J ChanG S Hayward

Abstract

Cytomegalovirus (CMV) infection is nonpermissive or persistent in many lymphoid and myeloid cell types but can be activated in differentiated macrophages. We have shown elsewhere that both the major immediate-early gene (MIE) and lytic cycle infectious progeny virus expression can be induced in otherwise nonpermissive monocyte-like U-937 cell cultures infected with either human CMV (HCMV) or simian CMV (SCMV) by treatment with the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA). Two multicopy basal enhancer motifs within the SCMV MIE enhancer, namely, 11 copies of the 16-bp cyclic AMP response element (CRE) and 3 copies of novel 17-bp serum response factor (SRF) binding sites referred to as the SNE (SRF/NFkappaB-like element), as well as four classical NFkappaB sites within the HCMV version, contribute to TPA responsiveness in transient assays in monocyte and T-cell types. The SCMV SNE sites contain potential overlapping core recognition binding motifs for SRF, Rel/NFkappaB, ETS, and YY1 class transcription factors but fail to respond to either serum or tumor necrosis factor alpha. Therefore, to evaluate the mechanism of TPA responsiveness of the SNE motifs and of a related 16-bp SEE (SRF/ETS element) motif found in th...Continue Reading

References

May 15, 1976·International Journal of Cancer. Journal International Du Cancer·C Sundström, K Nilsson
Feb 1, 1992·The Journal of General Virology·J H SinclairJ G Sissons
Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P GhazalR M Evans
Jul 1, 1992·Trends in Biochemical Sciences·K MacleodD Stehelin
Apr 1, 1992·Trends in Biochemical Sciences·V BlankA Israël
Sep 1, 1991·The Journal of General Virology·J Taylor-WiedemanJ H Sinclair
Dec 1, 1991·Journal of Virology·C E IbanezJ A Nelson
Jan 1, 1990·The Journal of General Virology·T StammingerB Fleckenstein
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·N K BhatT S Papas
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·P GhazalL Hennighausen
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·B A ChristyD Nathans
Oct 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·G P RiceM B Oldstone
Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski

❮ Previous
Next ❯

Citations

Dec 24, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hongkang Xi, Gilbert J Kersh
Feb 8, 2007·Cancer Science·Ryoko Utsubo-KuniyoshiKiyohiko Hatake
Jul 30, 2011·Future Virology·Christopher M TraylenShaw M Akula
Jan 21, 2003·The Journal of General Virology·Andrew J DavisonGary S Hayward
Apr 29, 2004·Journal of Virology·Philip E LashmitMark F Stinski
Feb 15, 2001·Oncogene·V I Sementchenko, D K Watson
Jun 30, 2006·The Journal of Biological Chemistry·Mohamed K El-TananiPhilip S Rudland
Mar 22, 2006·Expert Opinion on Therapeutic Targets·Chi-Chung WangPan-Chyr Yang
Jun 27, 2007·The Journal of Biological Chemistry·Anne FleigeAngela Schippers
Sep 1, 2010·Clinical Epigenetics·Georges Herbein, Daniel Wendling
Aug 8, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·J CinatlH W Doerr
Oct 30, 2020·Pathogens·Abigail L Dooley, Christine M O'Connor

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.