Synergistic neuroprotective effect of schisandrin and nootkatone on regulating inflammation, apoptosis and autophagy via the PI3K/AKT pathway

Food & Function
Yu QiYing Jia

Abstract

Alzheimer's disease (AD) is a neurodegenerative disease that seriously threatens elderly health. Schisandrin (SCH) and nootkatone (NKT) are two core components derived from Alpinia oxyphylla-Schisandra chinensis herb pair (ASHP), a traditional Chinese medicine formulation. Previous studies demonstrated that the combination of NKT and SCH exerted a neuroprotective effect in AD mouse models. The present study was undertaken to investigate whether there was a synergistic effect between NKT and SCH and the possible mechanism in Aβ1-42 induced PC12 cells. SCH (50 μM) and NKT (10 μM) had the most notable inhibitory effect on the level of Aβ secreted by cells. Treatment with NKT + SCH activated the PI3K/AKT/Gsk-3β/mTOR pathway. Inflammation related proteins such as NF-κB, IKK, IL-1β, IL-6 and TNF-α were decreased. The levels of cleaved-Caspase3 and LC3-II were reduced, indicating that apoptosis and autophagy were inhibited. These results revealed that NKT + SCH exerted a neuroprotective effect via the PI3K/AKT pathway, inhibiting inflammation, apoptosis and autophagy.

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Citations

Oct 2, 2020·International Journal of Molecular Sciences·Fang LuoXin Wang
Jan 1, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Jia LiZhi Sun
Jul 12, 2020·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Fatemeh AbbaszadehHaroon Khan
Apr 13, 2021·New Journal of Chemistry = Nouveau Journal De Chimie·Rawan AlnufaieMohammad A Alam
Aug 15, 2021·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sajad FakhriJianbo Xiao
Aug 28, 2021·International Journal of Molecular Sciences·Sachiko KoyamaThomas Heinbockel
Nov 7, 2021·Cellular and Molecular Life Sciences : CMLS·Rohan Gupta Pravir Kumar

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Methods Mentioned

BETA
protein extraction
Protein Assay
PCR
ELISA
nuclear translocation
Flow

Software Mentioned

ImageJ
CompuSyn
GraphPad Prism

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