PMID: 9525650Apr 3, 1998Paper

Synergistic neutralization of simian-human immunodeficiency virus SHIV-vpu+ by triple and quadruple combinations of human monoclonal antibodies and high-titer anti-human immunodeficiency virus type 1 immunoglobulins

Journal of Virology
A LiRuth M Ruprecht

Abstract

We have tested triple and quadruple combinations of human monoclonal antibodies (MAbs), which are directed against various epitopes on human immunodeficiency virus type 1 (HIV-1) envelope glycoproteins, and a high-titer anti-HIV-1 human immunoglobulin (HIVIG) preparation for their abilities to neutralize a chimeric simian-human immunodeficiency virus (SHIV-vpu+). This virus encodes the HIV-1 strain IIIB env, tat, rev, and vpu genes. The quantitative nature of the Chou-Talalay method (Adv. Enzyme Regul. 22:27-55, 1984) allows ranking of various combinations under identical experimental conditions. Of all triple combinations tested, the most potent neutralization was seen with MAbs 694/98D plus 2F5 plus 2G12 (directed against domains on V3, gp41, and gp120, respectively) as measured by the total MAb concentration required to reach 90% neutralization (90% effective concentration [EC90], 2.0 microg/ml). All triple combinations involving MAbs and/or HIVIG that were tested yielded synergy with combination index values of < 1; the dose reduction indices (DRIs) ranged from 3.1 to 26.2 at 90% neutralization. When four MAbs (the previous three plus MAb F105, directed against the CD4 binding site) were combined, higher neutralization pote...Continue Reading

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Citations

Nov 5, 2008·Expert Review of Vaccines·Sean P McBurney, Ted M Ross
Jun 15, 2007·Journal of Virology·Amapola ManriqueAlexandra Trkola
Sep 1, 2013·Pediatria polska·Leonard BothAnthony R Fooks
Aug 11, 2011·AIDS Research and Human Retroviruses·Weizao Chen, Dimiter S Dimitrov
Dec 11, 1999·Journal of Medical Primatology·N L Haigwood
Apr 14, 2017·Cell Reports·Katie A HowellM Javad Aman
Jun 29, 2002·Science·Brian GaschenBette Korber

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