Synergistic regulation of endothelial tight junctions by antioxidant (Se) and polyunsaturated lipid (GLA) via Claudin-5 modulation

Journal of Cellular Biochemistry
Tracey A MartinWen G Jiang

Abstract

Tight junctions (TJs) in endothelial cells act as cell-cell adhesion structures, governing paracellular permeability (PCP). Disruption can lead to leaky vascular bed and potentially to oedema and swelling of tissues, the aetiology of mastalgia. These changes may also cause vascular spread of cancer cells. This study aimed to determine whether the function of TJs in endothelial cells can be strengthened by gamma linolenic acid (GLA), selenium (Se) and iodine (I) in the presence of 17beta-estradiol (17beta-estradiol), which causes leakage of endothelial cells by disruption of TJs in endothelium. GLA, I, and Se individually increased transendothelial resistance. The combination of all three agents also had a significant effect on TER. Addition of GLA/Se/I reduced PCP of the endothelial cells. Treatment with GLA/Se/I reversed the effect of 17beta-estradiol in reducing TER and increasing PCP. Immunofluorescence revealed that after treatment with Se/I/GLA over 24 h there was increasing relocation to endothelial cell-cell junctions of the TJ proteins Claudin-5, Occludin, and ZO-1. Interestingly, this relocation was particularly evident with treatments containing I when probing with Claudin-5 and those containing Se for Occludin. There...Continue Reading

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Citations

Jan 18, 2011·Antioxidants & Redox Signaling·Ingolf E BlasigReiner F Haseloff
Oct 21, 2006·The Journal of Clinical Investigation·Tatiana KisselevaChristian Schindler
Apr 1, 2008·Annals of the New York Academy of Sciences·Emily VandenbrouckeAsrar B Malik
Apr 30, 2008·Journal of Cellular Biochemistry·Tracey A MartinWen G Jiang
Dec 9, 2008·Biochimica Et Biophysica Acta·Tracey A Martin, Wen G Jiang
Mar 18, 2008·Molecular & Cellular Proteomics : MCP·Erica M PasiniAlan W Thomas
May 9, 2012·Journal of Experimental & Clinical Cancer Research : CR·Astrid Escudero-EsparzaTracey A Martin
Nov 1, 2011·Molecular and Cellular Biochemistry·Astrid Escudero-EsparzaTracey A Martin
Feb 12, 2021·Metallomics : Integrated Biometal Science·Evgenii DrobyshevTanja Schwerdtle

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