PMID: 9436464Jun 1, 1997Paper

Synergy between CD28 and CD9 costimulation for naive T-cell activation

Immunology Letters
K Toyo-okaH Fujiwara

Abstract

Our previous study demonstrated that CD9 is expressed on most mature naive T-cells and delivers a potent costimulatory signal that functions independently of CD28. Here, we investigated whether this CD9-mediated signal is different from the CD28-mediated signal in the mode of costimulation and whether both signals function synergistically for T-cell activation. Anti-CD9 or anti-CD28 monoclonal antibody (mAb) increased [3H]TdR incorporation of naive T-cells in the absence of antigen-presenting cells (APC) when coimmobilized with submitogenic doses of anti-CD3 mAb. The levels of costimulation induced by ligation of CD9 and CD28 were comparable. However, the costimulatory effect differed between soluble anti-CD9 and CD28 mAb. A soluble form of anti-CD28 mAb could costimulate anti-CD3-triggered T-cells, whereas soluble anti-CD9 mAb failed to costimulate. Although anti-CD28 costimulated naive T-cells treated with phorbol myristate acetate (PMA) instead of anti-CD3 mAb, a combination of PMA plus anti-CD9 mAb could not induce T-cell activation. The combined costimulation of anti-CD3-triggered T-cells with anti-CD9 and anti-CD28 mAbs resulted in strikingly enhanced [3H]TdR uptake and lymphokine (IL-2 and IFN-gamma) production when comp...Continue Reading

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Citations

Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Yael SagiShoshana Levy
Sep 18, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sophie BrouardMinnie Sarwal
Nov 20, 2009·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Joseph PidalaClaudio Anasetti
Jan 10, 2018·Frontiers in Immunology·María Laura SaizFrancisco Sánchez-Madrid
Mar 31, 2021·Developmental and Comparative Immunology·Jemma V MilburnWilhelm Gerner

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