Synergy between EngE, XynA and ManA from Clostridium cellulovorans on corn stalk, grass and pineapple pulp substrates.

3 Biotech
B OlverBrett I Pletschke

Abstract

The synergistic interaction between various hemi/cellulolytic enzymes has become more important in order to achieve effective and optimal degradation of complex lignocellulose substrates for biofuel production. This study investigated the synergistic effect of three enzymes endoglucanase (EngE), mannanase (ManA) and xylanase (XynA) on the degradation of corn stalk, grass, and pineapple fruit pulp and determined the optimal degree of synergy between combinations of these enzymes. It was established that EngE was essential for degradation of all of the substrates, while the hemicellulases were able to contribute in a synergistic fashion to increase the activity on these substrates. Maximum specific activity and degree of synergy on the corn stalk and grass was found with EngE:XynA in a ratio of 75:25%, with a specific activity of 41.1 U/mg protein and a degree of synergy of 6.3 for corn stalk, and 44.1 U/mg protein and 3.4 for grass, respectively. The pineapple fruit pulp was optimally digested using a ManA:EngE combination in a 50:50% ratio; the specific activity and degree of synergy achieved were 52.4 U/mg protein and 2.7, respectively. This study highlights the importance of hemicellulases for the synergistic degradation of c...Continue Reading

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Citations

Apr 27, 2005·Journal of Bioinformatics and Computational Biology·Stephen RamseyHamid Bolouri
Sep 3, 2013·Applied Microbiology and Biotechnology·R J GruningerT A McAllister
Jun 13, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Jian-Rong CaiEn Han
Jan 15, 2014·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroyuki NakazawaRie Ito
Nov 15, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Karolina KucharskaMarian Kamiński

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Datasets Mentioned

BETA
AF105331
AF132735

Methods Mentioned

BETA
electrophoresis
protein assay

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