Synergy between the small intrinsically disordered protein Hsp12 and trehalose sustain viability after severe desiccation

ELife
Skylar Xantus KimHugo Tapia

Abstract

Anhydrobiotes are rare microbes, plants and animals that tolerate severe water loss. Understanding the molecular basis for their desiccation tolerance may provide novel insights into stress biology and critical tools for engineering drought-tolerant crops. Using the anhydrobiote, budding yeast, we show that trehalose and Hsp12, a small intrinsically disordered protein (sIDP) of the hydrophilin family, synergize to mitigate completely the inviability caused by the lethal stresses of desiccation. We show that these two molecules help to stabilize the activity and prevent aggregation of model proteins both in vivo and in vitro. We also identify a novel in vitro role for Hsp12 as a membrane remodeler, a protective feature not shared by another yeast hydrophilin, suggesting that sIDPs have distinct biological functions.

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Citations

Jan 10, 2020·Physiologia Plantarum·Akhilesh K Pandey, Arti Gautam
Mar 25, 2020·International Journal of Molecular Sciences·Arndt Wallmann, Christopher Kesten
Jul 3, 2020·Plants·Lynnette M A DirkAllan Bruce Downie
Jan 25, 2021·Protein Expression and Purification·Antoine LégerCharlotte Cabanne
Nov 17, 2020·Frontiers in Physiology·Jonathan D HibshmanBob Goldstein

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Methods Mentioned

BETA
gene knock-downs
NMR
electron microscopy
Circular dichroism
protein assay
Assay
PCR

Software Mentioned

STDEVP
Excel

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