Synergy of BID with doxorubicin in the killing of cancer cells

Oncology Reports
Emilia OrzechowskaJoanna Trzcińska-Danielewicz

Abstract

Overexpression of the BH3-interacting domain death agonist (BID) protein sensitizes certain cancer cell lines to apoptosis induced by anticancer agents, particularly by those acting through death receptors (e.g. TRAIL). Previously, we showed that recombinant BID fused with TAT cell penetrating peptide (TAT-BID) allowed for controlled delivery of BID to different cancer cell lines and moderately sensitized some of them to TRAIL or slightly to camptothecin. In the present study, we showed that TAT-BID delivered to HeLa cells strongly sensitized them to doxorubicin, as identified by cell viability and apoptosis assays. Another cell line sensitized to doxorubicin was PC3, whereas A549 and LNCaP cells were sensitized moderately or not at all, respectively. Sensitization was more pronounced at 1 µM doxorubicin administered for 48 h than for lower doses and shorter treatments. TAT-BID and doxorubicin may thus be considered as a potential therapeutic combination for cervical carcinoma and advanced prostate cancer treatment.

References

May 19, 2006·International Journal of Cancer. Journal International Du Cancer·Ji MiaoPaul B S Lai
Aug 17, 2010·Biochemical Pharmacology·S BienH K Kroemer
Nov 30, 2010·Cell Death and Differentiation·Y LiuS S Zinkel
Feb 8, 2011·Biochimica Et Biophysica Acta·Chahrazade Kantari, Henning Walczak
Oct 25, 2011·Journal of Radiation Research·Zhenhua HeLarry H Rohde
Dec 2, 2011·Journal of Immunotherapy·Takaya TsunoKathryn C Zoon
Jan 3, 2013·The Journal of Pharmacy and Pharmacology·Oktay TacarCrispin R Dass
Dec 24, 2013·Biochimica Et Biophysica Acta·Fan YangSteven Henikoff
Oct 19, 2014·BMC Cancer·Emilia Joanna OrzechowskaJoanna Trzcinska-Danielewicz

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Citations

Nov 20, 2019·International Journal of Molecular Sciences·Fuyan HuXiaoping Liu
Feb 28, 2020·Experimental and Therapeutic Medicine·Zheng XiaoXi Zeng
Sep 24, 2020·International Journal of Molecular Sciences·Kishu RanjanChandramani Pathak
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rui MedeirosFátima Cerqueira
Dec 26, 2018·ACS Applied Materials & Interfaces·Valentina BregaSamuel W Thomas

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Methods Mentioned

BETA
electrophoresis
confocal microscopy

Software Mentioned

Statistica
NIS
Elements

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