Synergy of interleukin 1 (IL-1) with arachidonic acid and A23187 in stimulating PGE synthesis in human fibroblasts: IL-1 stimulates fibroblast cyclooxygenase

Clinical Immunology and Immunopathology
J H KornS Y Ho

Abstract

The stimulation of prostaglandin E (PGE) synthesis by interleukin 1 (IL-1) has important physiologic effects in many target tissues. The mechanism(s) whereby IL-1 stimulates PGE synthesis is not well understood. Two alternative mechanisms have been postulated: hydrolysis of membrane phospholipid with release of arachidonic acid substrate and induction of cyclooxygenase activity. We examined these mechanisms in IL-1 stimulation of human dermal fibroblast PGE synthesis. IL-1 failed to induce release of previously incorporated radiolabeled arachidonic acid from fibroblasts under conditions where there was a 30-fold or greater stimulation of PGE synthesis. The calcium ionophore, A23187, gave much less stimulation of PGE synthesis while inducing 30-100% release of incorporated [14C]arachidonic acid. There was marked synergy between IL-1 and agents which increased availability of arachidonic acid. For example, the combination of IL-1 and A23187, used at concentrations where neither agent alone stimulated PGE synthesis, increased PGE synthesis from 3.1 to 22.5 ng/ml; similar synergy was seen between IL-1 and exogenous arachidonic acid. To examine a possible effect of IL-1 on cyclooxygenase synthesis, fibroblast cyclooxygenase was meas...Continue Reading

References

Nov 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·L M WahlS E Mergenhagen
Feb 1, 1976·Biochemical Pharmacology·R J Flower, G J Blackwell
Dec 1, 1984·The Journal of Clinical Investigation·P J Whiteley, P Needleman
Jan 1, 1980·Clinical Immunology and Immunopathology·J S Goodwin, D R Webb

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Citations

Jul 1, 1997·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·C A TerreginoG J Slotman
Apr 22, 1992·European Journal of Pharmacology·G R ElliottJ Van Dijk
Aug 1, 1991·The Journal of Investigative Dermatology·H H Henneicke-von ZepelinE Christophers
Jan 1, 1994·The Journal of Investigative Dermatology·N GodessartJ M de Moragas
Mar 1, 1991·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·M SaitoZ Davidovitch

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