PMID: 40118Jan 1, 1979

Syneristic and antagonistic effects of glucocorticoids on insulin action

Monographs on Endocrinology
T D Gelehrter

Abstract

HTC cells, an established line of rat hepatoma cells in tissue culture, provide a useful experimental model system for studying the interaction of glucocorticoids and insulin in the regulation of protein metabolism. The actions of insulin and glucocorticoids on amino acid transport and protein degradation are antagonistic in this cell line. In contrast, the actions of these two hormones are additive with regard to the induction of tyrosine aminotransferase. The addition of insulin to HTC cells previously incubated with dexamethasone causes a rapid further doubling in the cellular concentration of this enzyme. The properties of the induction by insulin differ in several respects from the induction by glucocorticoids. The former occurs immediately, without the characteristic lag observed during induction by steroids. Insulin induction of transaminase does not require concomitant RNA synthesis, and does not cause the accumulation of specific mRNA for this enzyme as do glucocorticoids. Using specific immunoprecipitation techniques, we have demonstrated that insulin stimulates a nonselective increase in the rate of total protein synthesis in HTC cells, and a selective decrease in the rate of degradation of tyrosine aminotransferase ...Continue Reading

Related Concepts

Protein Degradation, Regulatory
Insulin Antagonists
Glucocorticoid inhalants for obstructive airway disease
RNA Chemical Synthesis
Liver Carcinoma
Tyrosine Aminotransferase
Protein Degradation, Metabolic
Glucocorticoids, Systemic
Hepatoma, Novikoff
Novolin

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