Synovial cell culture and tissue engineering of a tendon synovial cell biomembrane

Journal of Biomedical Materials Research. Part a
Akif Muhtar OzturkAgnes Tan

Abstract

Restrictive adhesions are a common complication of tendon injury and repair in the hand, resulting in severe dysfunction. Creating a barrier between the repair sites and surrounding tissue layers may prevent adhesions. We present the first stage in the process of developing a synovial biomembrane for this purpose. Synovial cells harvested from the Achilles tendon sheath and the knee joint of a Wistar albino rat were cultured for 2 weeks in culture medium, and then impregnated into a collagen type 1 matrix for another 2 weeks. Cells originating from both tendon and synovium demonstrated cell growth and layer formation on the surfaces of the matrix 2 weeks after impregnation. Alcian blue staining using Scott's method demonstrated the presence of acidic mucopolysaccharide, indicating hyaluronic acid (HA) production. This provides indirect evidence of functioning synovial cells on the membrane. It is possible to culture synovial cells and engineer a synoviocyte-collagen membrane that synthesizes endogenous HA. Application of this biomembrane to tendon repair sites may help to prevent adhesions after tendon repairs. Evaluation of this method on in vivo models is required.

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Citations

Jul 3, 2010·Plastic and Reconstructive Surgery·Lu Zhang, Myron Spector
Mar 31, 2015·International Journal of Molecular Sciences·Shichao JiangCunyi Fan
Feb 23, 2010·Biomaterials·Liang XuYilin Cao
Apr 11, 2012·Journal of Biomedical Materials Research. Part a·Gang ChenZheng-Zhi Zhang
Apr 9, 2013·Journal of Biomedical Materials Research. Part a·Ke JiangYan Xiong
Mar 13, 2014·Journal of Biomedical Materials Research. Part a·Alim Can BaymuratDeniz Erdogan
Nov 23, 2013·The Journal of Hand Surgery, European Volume·M G GalvezJ Chang
Mar 17, 2015·The Journal of Hand Surgery, European Volume·E KloczkoL Yildirimer
Feb 7, 2009·Tissue Engineering. Part B, Reviews·Jiabing FanDong-An Wang
Sep 28, 2016·Plastic and Reconstructive Surgery·Kai MegerleJames Chang

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