Syntaxin 1A is required for normal in utero development

Biochemical and Biophysical Research Communications
John E McRoryGerald W Zamponi

Abstract

We have generated a syntaxin 1A knockout mouse by deletion of exons 3 through 6 and a concomitant insertion of a stop codon in exon 2. Heterozygous knockout animals were viable with no apparent phenotype. In contrast, the vast majority of homozygous animals died in utero, with embryos examined at day E15 showing a drastic reduction in body size and development when compared to WT and heterozygous littermates. Surprisingly, out of a total of 204 offspring from heterozygous breeding pairs only four homozygous animals were born alive and viable. These animals exhibited reduced body weight, but showed only mild behavioral deficiencies. Taken together, our data indicate that syntaxin 1A is an important regulator of normal in utero development, but may not be essential for normal brain function later in life.

References

Jan 1, 1997·The Biochemical Journal·M N JagadishC W Ward
Sep 10, 1998·Nature Structural Biology·M A PoirierM K Bennett
Aug 17, 1999·The EMBO Journal·I DulubovaJ Rizo
Nov 23, 2000·Proceedings of the National Academy of Sciences of the United States of America·I BezprozvannyR W Tsien
Sep 11, 2002·The Journal of Biological Chemistry·Scott E JarvisGerald W Zamponi
Feb 28, 2003·Journal of Neuroscience Methods·Jennifer L Tillerson, Gary W Miller
Apr 3, 2003·The European Journal of Neuroscience·Elise F Stanley
Jul 10, 2003·Current Opinion in Neurobiology·J David Spafford, Gerald W Zamponi
May 26, 2006·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Tomonori FujiwaraKimio Akagawa
Oct 4, 2006·Proceedings of the National Academy of Sciences of the United States of America·Paola SgadòHorst H Simon

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Citations

May 4, 2010·Human Genetics·Giuseppe MerlaCarmela Fusco
Dec 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Massimiliano AgostiniGerry Melino
Sep 26, 2009·Molecular Human Reproduction·E Rajcan-SeparovicT Philipp
Feb 10, 2016·Journal of Neural Transmission·Renata Basso CupertinoNina Roth Mota
Jul 19, 2011·Biochemical and Biophysical Research Communications·Jonathan N DaviesGerald W Zamponi
May 11, 2011·The FEBS Journal·Kathleen Zylbersztejn, Thierry Galli
Apr 29, 2010·American Journal of Medical Genetics. Part C, Seminars in Medical Genetics·Lucy R Osborne
Jul 22, 2014·Progress in Neurobiology·Stephen D MerineyCameron B Gundersen
Dec 22, 2017·Journal of Intellectual Disability Research : JIDR·L CastigliaE G Coci
Sep 23, 2020·Journal of Cellular Physiology·Bor L Tang
Feb 24, 2017·Neurogenesis·Thanathom Chailangkarn, Alysson R Muotri

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