Syntaxin 4 regulates the surface localization of a promyogenic receptor Cdo thereby promoting myogenic differentiation

Skeletal Muscle
Miran YooGyu-Un Bae

Abstract

Syntaxins are a family of membrane proteins involved in vesicle trafficking, such as synaptic vesicle exocytosis. Syntaxin 4 (Stx4) is expressed highly in skeletal muscle and plays a critical role in insulin-stimulated glucose uptake by promoting translocation of glucose transporter 4 (GLUT4) to the cell surface. A cell surface receptor cell adhesion molecule-related, down-regulated by oncogenes (Cdo) is a component of cell adhesion complexes and promotes myoblast differentiation via activation of key signalings, including p38MAPK and AKT. In this study, we investigate the function of Stx4 in myoblast differentiation and the crosstalk between Stx4 and Cdo in myoblast differentiation. The effects of overexpression or shRNA-based depletion of Stx4 and Cdo genes on C2C12 myoblast differentiation are assessed by Western blotting and immunofluorescence approaches. The interaction between Cdo and Stx4 and the responsible domain mapping are assessed by coimmunoprecipitation or pulldown assays. The effect of Stx4 depletion on cell surface localization of Cdo and GLUT4 in C2C12 myoblasts is assessed by surface biotinylation and Western blotting. Overexpression or knockdown of Stx4 enhances or inhibits myogenic differentiation, respectiv...Continue Reading

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Citations

Feb 26, 2016·Journal of Molecular and Cellular Cardiology·Myong-Ho JeongJong-Sun Kang
Feb 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·Myong-Ho JeongJong-Sun Kang

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Methods Mentioned

BETA
transfection
Reverse Transcription Polymerase Chain Reaction
immunoprecipitation
Electrophoresis
pulldown
PCR
two-hybrid
pulled down
confocal microscopy
GTPases

Software Mentioned

Elements F
NIS
ABI 7000
Image Gauge

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