Syntaxin 7, a novel syntaxin member associated with the early endosomal compartment.

The Journal of Biological Chemistry
S H WongW Hong

Abstract

Members of the syntaxin family are key molecules involved in diverse vesicle docking/fusion events. We report here the molecular, biochemical, and cell biological characterizations of a novel member (syntaxin 7) of the syntaxin family. Syntaxin 7 is structurally related to all known syntaxins. Within a 79-residue region preceding the C-terminal hydrophobic tail, syntaxin 7 is 35, 34, 34, 34, 25, and 19% identical to syntaxins 1, 2, 3, 4, 5, and 6, respectively. Northern blot analysis showed that syntaxin 7 is widely expressed. Indirect immunofluorescence microscopy revealed that syntaxin 7 is primarily associated with the early endosome. In vitro binding assays established that syntaxin 7 in membrane extracts interacts with immobilized recombinant alpha-soluble N-ethylmaleimide-sensitive factor attachment proteins fused to glutathione S-transferase. Our results highlight the general importance of members of the syntaxin family in protein trafficking and provide new avenues for future functional and mechanistic studies of this first endosomal syntaxin as well as the endocytotic pathway.

References

Oct 1, 1992·The Journal of Cell Biology·S A Wood, W J Brown
Jan 1, 1992·Annual Review of Biochemistry·N K PryerR Schekman
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Nov 1, 1991·The Journal of Cell Biology·J Tooze, M Hollinshead
Jun 1, 1991·Journal of Biochemistry·K WatanabeY Arai
May 24, 1991·Science·A LupasJ Stock
Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·G LiP D Stahl
Nov 24, 1995·The Journal of Biological Chemistry·S H HansenJ E Casanova
Oct 1, 1995·The Biochemical Journal·A T Jones, M J Clague
Jul 3, 1995·FEBS Letters·M J ClagueA T Jones
Sep 10, 1993·Cell·M K BennettR H Scheller
Jun 15, 1995·Biochemical and Biophysical Research Communications·P R ShepherdK Siddle
Feb 3, 1995·The Journal of Biological Chemistry·H T McMahon, T C Südhof
Nov 3, 1994·Nature·J E Rothman
Jul 21, 1994·Nature·S Ferro-Novick, R Jahn
Sep 15, 1993·Biochemical and Biophysical Research Communications·F KanaiY Ebina
Mar 25, 1993·Nature·T SöllnerJ E Rothman
Dec 1, 1995·Cell·J A WhitneyI Mellman
Mar 15, 1996·Science·R Schekman, L Orci
Apr 12, 1996·Science·J E Rothman, F T Wieland
Jul 26, 1996·The Journal of Biological Chemistry·J B BockR H Scheller
Jan 1, 1996·Methods in Enzymology·A Lupas
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·E DaroI Mellman
Dec 1, 1996·Molecular Biology of the Cell·S H LowK E Mostov
Dec 1, 1996·Molecular Biology of the Cell·H Y GaisanoW S Trimble
May 8, 1997·Nature·J B Bock, R H Scheller

❮ Previous
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Citations

Apr 16, 2002·Molecular Biology of the Cell·Martin SachseJudith Klumperman
Nov 17, 1999·Molecular Biology of the Cell·R PrekerisR H Scheller
Aug 6, 1999·Molecular Biology of the Cell·S D Conner, G M Wessel
Sep 3, 1999·Molecular Biology of the Cell·C HolroydR Jahn
Jul 16, 2008·Molecular Biology and Evolution·Tobias H KloepperDirk Fasshauer
Jun 29, 2000·Annual Review of Biochemistry·R Jahn, T C Südhof
Jun 2, 2000·Biochemical and Biophysical Research Communications·R MinakamiH Sugiyama
May 20, 1999·Molecular Membrane Biology·I G MillsM J Clague
May 20, 1999·Molecular Membrane Biology·K Mohrmann, P van der Sluijs
Nov 17, 1998·The Journal of Cell Biology·R PrekerisR H Scheller
Aug 25, 1999·The Journal of Cell Biology·R J AdvaniR H Scheller
Feb 23, 2010·Immunology Letters·Li-xin XieFaustino Mollinedo
Oct 30, 2007·Cellular & Molecular Biology Letters·Kazuo KasaiKimio Akagawa
May 10, 2012·Journal of Proteomics·Domitille SchvartzJean-Charles Sanchez
Aug 31, 2000·Annual Review of Biochemistry·W Wickner, A Haas
Jun 6, 2000·Neuron·S J ScalesR H Scheller
Feb 27, 1999·Experimental Cell Research·H R Pelham
Sep 15, 2004·Developmental Cell·Cheng-Chun WangWanjin Hong
Mar 15, 2008·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Mayumi Nakanishi-Matsui, Masamitsu Futai
Oct 3, 1999·The Journal of Biological Chemistry·A SimonsenH Stenmark
Jan 8, 1999·Molecular Biology of the Cell·S H WongW Hong
Jul 11, 2000·The European Journal of Neuroscience·H HirlingS Catsicas
Dec 16, 1998·The Journal of Biological Chemistry·M SteegmaierR H Scheller
Apr 18, 1998·The Journal of Biological Chemistry·B L TangW Hong
Feb 29, 2000·The Journal of Biological Chemistry·N NakamuraM Futai
Sep 10, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Richard F CollinsWilliam S Trimble
Jan 15, 2011·Molecular Membrane Biology·Deyu Tarika CaiSiew Heng Wong
Aug 29, 2009·Journal of Proteome Research·Sara StrömbergFredrik Ponten
Nov 5, 2002·Journal of Cell Science·Hao XuWilliam S Trimble

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