Syntenin is involved in the bacteria clearance response of kuruma shrimp (Marsupenaeus japonicus)

Fish & Shellfish Immunology
Qian LiuCui-jie Kang

Abstract

Syntenin is a multifunctional cytosolic adaptor protein that contributes to cell migration, proliferation, attachment, and apoptosis, as well as immune response to virus, in vertebrates. However, the functions of syntenin in the antibacterial response of invertebrates remain unclear. In this study, we identified a syntenin-like gene (MjSyn) from the kuruma shrimp (Marsupenaeus japonicus) and detected its function in the antibacterial immunity of shrimp. The full-length MjSyn was 1223 bp with a 963 bp open reading frame that encodes 320 amino acids. The deduced MjSyn proteins contained two atypical PDZ domains (sequence repeat that was first reported in the postsynaptic density protein or PSD-95, DlgA, and ZO-1 protein), an N-terminal domain, and a C-terminal domain. Reverse transcription (RT)-PCR results showed that MjSyn was expressed in all tested tissues. Quantitative real-time PCR analysis revealed that MjSyn transcripts in the hemocyte, gill, and intestine were significantly induced at various time points after infection with Staphylococcus aureus and Vibrio anguillarum. The knockdown of the expression of MjSyn by RNA interference resulted in a significant decrease in the phagocytic ability and increased bacteria number in ...Continue Reading

References

Aug 15, 1996·Nature·J H Morais CabralR C Liddington
Feb 12, 1998·Proceedings of the National Academy of Sciences of the United States of America·J J GrootjansG David
Feb 12, 1998·Science·T Pawson, J D Scott
Mar 17, 1999·The Journal of Clinical Investigation·A S Fanning, J M Anderson
Jan 16, 2002·Current Opinion in Immunology·Steven Greenberg, Sergio Grinstein
Apr 8, 2003·Structure·Beom Sik KangZygmunt S Derewenda
Dec 31, 2003·Molecular Immunology·Bruce Beutler
Nov 3, 2004·Pharmacology & Therapeutics·Devanand SarkarPaul B Fisher
Mar 31, 2005·Molecular and Cellular Neurosciences·Hélène HirbecJeremy M Henley
Dec 3, 2005·Cancer Research·Habib BoukerchePaul B Fisher
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 22, 2007·Science·Elodie GhedinAlan L Scott
Apr 25, 2008·Journal of Cell Science·Jeffrey M Beekman, Paul J Coffer
Apr 13, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Hee Jeong KongChang Hoon Lee
Nov 26, 2010·Current Opinion in Cell Biology·Lori A Rutkevich, David B Williams
Mar 2, 2011·Molecular and Cellular Biochemistry·Fumihiko OkumuraShigetsugu Hatakeyama
Feb 22, 2012·Journal of Cell Science·Mónica Sala-ValdésMaría Yáñez-Mó
Apr 27, 2012·Molecular Biology of the Cell·Mónica Gordón-AlonsoFrancisco Sánchez-Madrid
Jun 5, 2012·Nature Cell Biology·Maria Francesca BaiettiGuido David
May 27, 2014·Developmental and Comparative Immunology·Qing ZhangJin-Xing Wang

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Datasets Mentioned

BETA
KP241935
BC044454.1
AF000652.1

Methods Mentioned

BETA
Reverse transcription PCR
PCR

Software Mentioned

MEGA
Blastp
GraphPad Prism
MODEL
Clustal X
BLAST
PyMOL
SWISS

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