Synteny-Based Development of CAPS Markers Linked to the Sweet kernel LOCUS, Controlling Amygdalin Accumulation in Almond (Prunus dulcis (Mill.) D.A.Webb)

Genes
F RicciardiC Lotti

Abstract

The bitterness and toxicity of wild-type seeds of Prunoideae is due to the cyanogenic glucoside amygdalin. In cultivated almond (Prunus dulcis (Mill.) D.A. Webb), a dominant mutation at the Sk locus prevents amygdalin accumulation and thus results in edible sweet kernels. Here, we exploited sequence similarity and synteny between the genomes of almond and peach (Prunus persica (L.) Batsch) to identify cleaved amplified polymorphic sequence (CAPS) molecular markers linked to the Sk locus. A segregant F₁ population was used to map these markers on the Sk genomic region, together with Sk-linked simple sequence repeat (SSR) markers previously described. Molecular fingerprinting of a cultivar collection indicated the possibility to use CAPS polymorphisms identified in this study in breeding programs arising from different parental combinations. Overall, we highlight a set of codominant markers useful for early selection of sweet kernel genotypes, an aspect of primary importance in almond breeding. In addition, by showing collinearity between the physical map of peach and the genetic map of almond with respect to the Sk genomic region, we provide valuable information for further marker development and Sk positional cloning.

Citations

Feb 5, 2000·Nature Biotechnology·M Schuelke
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·E DirlewangerF Laigret
Jan 31, 2004·Phytochemistry·Mika ZagrobelnyBirger Lindberg Møller
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Feb 23, 2012·Plant Physiology·Raquel Sánchez-PérezKirsten Jørgensen
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Aug 26, 2016·Genetics and Molecular Biology·Maria Lucia Carneiro VieiraCarla de Freitas Munhoz

Related Concepts

Biological Markers
Gene Polymorphism
Study
Short Tandem Repeat
Vitamin B17 Measurement
Prunus persica
Genome
Cleaved
Tumor Markers, Biological
Chromosome Map

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