Synthesis and accumulation of cyanophycin in transgenic strains of Saccharomyces cerevisiae.

Applied and Environmental Microbiology
Anna SteinleAlexander Steinbüchel

Abstract

Cyanophycin [multi-L-arginyl-poly(L-aspartic acid) (CGP)] was, for the first time, produced in yeast. As yeasts are very important production organisms in biotechnology, it was determined if CGP can be produced in two different strains of Saccharomyces cerevisiae. The episomal vector systems pESC (with the galactose-inducible promoter GAL1) and pYEX-BX (with the copper ion-inducible promoter CUP1) were chosen to express the cyanophycin synthetase gene from the cyanobacterium Synechocystis sp. strain PCC 6308 (cphA(6308)) in yeast. Expression experiments with transgenic yeasts revealed that the use of the CUP1 promoter is much more efficient for CGP production than the GAL1 promoter. As observed by electrophoresis of isolated CGP in sodium dodecyl sulfate-polyacrylamide gels, the yeast strains produced two different types of polymer: the water-soluble and the water-insoluble CGP were observed as major and minor forms of the polymer, respectively. A maximum CGP content of 6.9% (wt/wt) was detected in the cells. High-performance liquid chromatography analysis showed that the isolated polymers consisted mainly of the two amino acids aspartic acid and arginine and that, in addition, a minor amount (2 mol%) of lysine was present. Gro...Continue Reading

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Citations

Sep 4, 2009·Applied Microbiology and Biotechnology·Anna Steinle, Alexander Steinbüchel
Oct 13, 2010·Applied Microbiology and Biotechnology·Yasser Abd-El-KaremAlexander Steinbüchel
Oct 6, 2010·Applied Microbiology and Biotechnology·Jens KrollAlexander Steinbüchel
Dec 12, 2012·Applied Microbiology and Biotechnology·Katja AdamesAlexander Steinbüchel
Dec 30, 2009·Applied and Environmental Microbiology·Anna SteinleAlexander Steinbüchel
Sep 4, 2012·Applied and Environmental Microbiology·Steffen LütteBärbel Friedrich
Oct 6, 2011·Applied Microbiology and Biotechnology·Bas J MeussenLeo H de Graaff
Dec 15, 2015·Applied Microbiology and Biotechnology·Henrik NauschInge Broer
Apr 20, 2011·New Biotechnology·Daniel K Y SolaimanAlexander Steinbüchel
Nov 27, 2015·Microbial Cell Factories·Björn WatzerKarl Forchhammer
Dec 14, 2017·Critical Reviews in Biotechnology·Matthias RabergAlexander Steinbüchel
Feb 11, 2014·Applied and Environmental Microbiology·Maja FrommeyerAlexander Steinbüchel

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