Synthesis and assessment of the relative toxicity of the oxidised derivatives of campesterol and dihydrobrassicasterol in U937 and HepG2 cells

Biochimie
Yvonne O'CallaghanNora M O'Brien

Abstract

The cytotoxic effects of the oxidised derivatives of the phytosterols, stigmasterol and β-sitosterol, have previously been shown to be similar but less potent than those of the equivalent cholesterol oxides in the U937 cell line. The objective of the present study was to compare the cytotoxic effects of the oxidised derivatives of synthetic mixtures of campesterol and dihydrobrassicasterol in both the U937 and HepG2 cell lines. The parent compounds consisted of a campesterol: dihydrobrassicasterol mix at a ratio of 2:1 (2CMP:1DHB) and a dihydrobrassicasterol:campesterol mix at a ratio of 3:1 (3DHB:1CMP). The 2CMP:1DBH oxides were more cytotoxic in the U937 cells than the 3DBH:1CMP oxides but the difference in cytotoxicity was less marked in the HepG2 cells. The order of toxicity of the individual oxidation products was found to be similar to that previously observed for cholesterol, β-sitosterol and stigmasterol oxidation products in the U937 cell line. There was an increase in apoptotic nuclei in U937 cells incubated with the 7-keto and 7β-OH derivatives of both 2CMP:1DHB and 3DHB:1CMP and also in the presence of 3DHB:1CMP-3β,5α,6β-triol and 2CMP:1DHB-5β,6β-epoxide. An additional oxidation product synthesised from 2CMP:1DHB, 5...Continue Reading

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Citations

Nov 26, 2013·Atherosclerosis·Rgia A OthmanPeter J H Jones
Feb 5, 2014·Biochemical and Biophysical Research Communications·Yvonne O'CallaghanNora M O'Brien
Feb 6, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Naiyer ShahzadSaeed Saeed Al-Ghamdi
Mar 20, 2015·Molecular Nutrition & Food Research·Birgit ScholzPablo Steinberg
Sep 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jingnan ChenYanlan Bi
Feb 23, 2021·Critical Reviews in Food Science and Nutrition·Simin FengChung S Yang
Nov 18, 2021·Chirality·Miguel Á Fuentes-FigueroaEleuterio Burgueño-Tapia

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