Synthesis and biocompatibility of a biodegradable and functionalizable thermo-sensitive hydrogel

Regenerative Biomaterials
Mantosh K SinhaYadong Wang

Abstract

Injectable thermal gels are a useful tool for drug delivery and tissue engineering. However, most thermal gels do not solidify rapidly at body temperature (37°C). We addressed this by synthesizing a thermo-sensitive, rapidly biodegrading hydrogel. Our hydrogel, poly(ethylene glycol)-co-poly(propanol serinate hexamethylene urethane) (EPSHU), is an ABA block copolymer comprising A, methoxy poly ethylene glycol group and B, poly (propanol L-serinate hexamethylene urethane). EPSHU was characterized by gel permeation chromatography for molecular weight and (1)H NMR and Fourier transformed infrared for structure. Rheological studies measured the phase transition temperature. In vitro degradation in cholesterol esterase and in Dulbecco's phosphate buffered saline (DPBS) was tracked using the average molecular weight measured by gel permeation chromatography. LIVE/DEAD and resazurin reduction assays performed on NIH 3T3 fibroblasts exposed to EPSHU extracts demonstrated no cytotoxicity. Subcutaneous implantation into BALB/cJ mice indicated good biocompatibility in vivo. The biodegradability and biocompatibility of EPSHU together make it a promising candidate for drug delivery applications that demand carrier gel degradation within months.

References

Jun 6, 2000·Advanced Drug Delivery Reviews·E S Ron, L E Bromberg
Jun 8, 2001·Journal of Controlled Release : Official Journal of the Controlled Release Society·G M ZentnerS Weitman
Jul 31, 2003·Journal of Biomedical Materials Research. Part a·Ivan AlferievRobert J Levy
May 4, 2004·Biomaterials·Kimberly A WoodhouseCatherine M Bellingham
Dec 29, 2004·International Journal of Pharmaceutics·Sibao ChenJagdish Singh
Sep 15, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·M T Khorasani, S Shorgashti
Nov 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kiyohaya ObaraTadaaki Maehara
Oct 20, 2006·International Journal of Pharmaceutics·Woo Sun ShimDoo Sung Lee
Feb 6, 2007·Acta Biomaterialia·Amber E RydholmChristopher N Bowman
Aug 7, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Michihiro OguraMasayoshi Watanabe
Mar 7, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chaoliang HeDoo Sung Lee
Sep 13, 2008·International Journal of Pharmaceutics·Yu Tang, Jagdish Singh
Aug 27, 2009·Biomacromolecules·Gusphyl Justin, Anthony Guiseppi-Elie
Mar 3, 2010·Macromolecular Bioscience·Minh Khanh Nguyen, Doo Sung Lee
Jul 25, 2012·Drug Delivery·Hitendra S MahajanPankaj Nerkar
Dec 29, 2013·Investigative Ophthalmology & Visual Science·Britta M RauckYadong Wang

❮ Previous
Next ❯

Citations

Oct 25, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaochu Ding, Yadong Wang
Aug 7, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xian Li, Xiulan Su

❮ Previous
Next ❯

Methods Mentioned

BETA
light scattering
column chromatography

Software Mentioned

NIH Image J
Gen5

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.