Synthesis and characterisation of novel chromogenic substrates for human pancreatic alpha-amylase

Carbohydrate Research
Iben DamagerStephen G Withers

Abstract

Derivatives of maltose and maltotriose were chemically synthesised as substrates for human pancreatic alpha-amylases and subjected to kinetic analysis. Rates measured were shown to reflect both hydrolysis and transglycosylation reactions. 4-O-Methylated derivatives of these substrates underwent only hydrolysis, thereby simplifying kinetic analyses. These modified substrates may be used for the detection and kinetic analysis of alpha-amylases, and are useful in rapidly screening for novel alpha-amylase inhibitors and for subsequent kinetic characterisation.

Citations

Apr 19, 2011·Journal of Agricultural and Food Chemistry·Yeyi GuJoshua D Lambert
Sep 15, 2011·The Journal of Biological Chemistry·Karl SysonStephen Bornemann
Nov 28, 2013·Carbohydrate Polymers·Chao LiYoung-Wan Kim
Sep 11, 2016·Materials Science & Engineering. C, Materials for Biological Applications·Céline Aubrun-FillâtrePhilippe Hivart
Mar 21, 2007·Chembiochem : a European Journal of Chemical Biology·Hong-Ming Chen, Stephen G Withers
Jun 25, 2021·Chemical Science·Ryan P SweeneyStephen G Withers
Feb 3, 2012·Journal of the American Chemical Society·Ethan D Goddard-BorgerStephen G Withers
May 3, 2014·Journal of Agricultural and Food Chemistry·Anna PodsędekMaria Koziołkiewicz
Feb 23, 2010·Bioorganic & Medicinal Chemistry Letters·Hiroyuki OkaKoji Matsuoka

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