Synthesis and characterization of a native, oligomeric form of recombinant severe acute respiratory syndrome coronavirus spike glycoprotein

Journal of Virology
Hyun Chul SongJang H Han

Abstract

We have expressed and characterized the severe acute respiratory syndrome coronavirus (SARS-CoV) spike protein in cDNA-transfected mammalian cells. The full-length spike protein (S) was newly synthesized as an endoglycosidase H (endo H)-sensitive glycoprotein (gp170) that is further modified into an endo H-resistant glycoprotein (gp180) in the Golgi apparatus. No substantial proteolytic cleavage of S was observed, suggesting that S is not processed into head (S1) and stalk (S2) domains as observed for certain other coronaviruses. While the expressed full-length S glycoprotein was exclusively cell associated, a truncation of S by excluding the C-terminal transmembrane and cytoplasmic tail domains resulted in the expression of an endoplasmic reticulum-localized glycoprotein (gp160) as well as a Golgi-specific form (gp170) which was ultimately secreted into the cell culture medium. Chemical cross-linking, thermal denaturation, and size fractionation analyses suggested that the full-length S glycoprotein of SARS-CoV forms a higher order structure of approximately 500 kDa, which is consistent with it being an S homotrimer. The latter was also observed in purified virions. The intracellular form of the C-terminally truncated S protei...Continue Reading

References

Oct 1, 1986·The Journal of Cell Biology·C S CopelandA Helenius
Dec 1, 1986·Journal of Virology·R W Doms, A Helenius
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·J M PoloT W Dubensky
Apr 12, 2003·The New England Journal of Medicine·Thomas G KsiazekUNKNOWN SARS Working Group
May 6, 2003·Science·Marco A MarraRachel L Roper
May 31, 2003·Science·Martin Enserink
Sep 23, 2003·BMC Microbiology·Yossef Kliger, Erez Y Levanon
Oct 31, 2003·Nature·Byron E E MartinaAlbert D M E Osterhaus
Dec 4, 2003·Science·Markus EickmannRino Rappuoli
Dec 4, 2003·Biochemical and Biophysical Research Communications·Xiaodong XiaoDimiter S Dimitrov
Dec 13, 2003·The Journal of Biological Chemistry·Swee Kee WongMichael Farzan
Feb 10, 2004·Biochemical and Biophysical Research Communications·Peigang WangHongkui Deng

❮ Previous
Next ❯

Citations

Jul 18, 2006·Nature Structural & Molecular Biology·Daniel R BeniacTim F Booth
Mar 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Sandrine BelouzardGary R Whittaker
Aug 24, 2005·Virology Journal·Martin J VincentStuart T Nichol
Aug 23, 2005·Emerging Infectious Diseases·Konrad StadlerKanta Subbarao
Apr 10, 2007·Virus Research·Luis EnjuanesRalph Baric
Jun 29, 2006·Cellular Microbiology·Cornelis A M de Haan, Peter J M Rottier
Jun 9, 2005·Biochemical and Biophysical Research Communications·Jinzhu DuanGang Jin
Sep 30, 2005·Vaccine·Deborah R Taylor
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Jung-Eun ParkTom Gallagher
Sep 1, 2016·Annual Review of Virology·Fang Li
Dec 15, 2004·Biochemical and Biophysical Research Communications·Eric BergeronNabil G Seidah
May 28, 2019·Virology Journal·Dewald Schoeman, Burtram C Fielding
Apr 21, 2020·Journal of Biomolecular Structure & Dynamics·Subramanian BoopathiPonmalai Kolandaivel
Oct 14, 2008·Experimental Biology and Medicine·Che-Man ChanKwok-Yung Yuen
Jul 23, 2020·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Jatin MachhiBhavesh D Kevadiya
Jun 22, 2019·Annual Review of Microbiology·To Sing Fung, Ding Xiang Liu
May 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Noor RahmanHaroon Khan
Jun 1, 2018·Future Virology·To Sing Fung, Ding Xiang Liu
Nov 11, 2020·Drug Development Research·Brilliant N MarakVed Prakash Singh

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Biochemical and Biophysical Research Communications
Xiaodong XiaoDimiter S Dimitrov
Proceedings of the National Academy of Sciences of the United States of America
Himani BishtBernard Moss
Proceedings of the National Academy of Sciences of the United States of America
Graham SimmonsPaul Bates
The New England Journal of Medicine
Thomas G KsiazekSARS Working Group
© 2021 Meta ULC. All rights reserved