Synthesis and characterization of a novel peptide-grafted Cs and evaluation of its nanoparticles for the oral delivery of insulin, in vitro, and in vivo study

International Journal of Nanomedicine
Ghullam Reza BarbariM Rafiee Tehrani

Abstract

Despite years of experience and rigorous research, injectable insulin is the sole trusted treatment method to control the blood glucose level in diabetes type 1 patients, but injection of insulin is painful and poses a lot of stress to the patients, especially children, therefore, development of a non-injectable formulation of insulin is a major breakthrough in the history of medicine and pharmaceutical sciences. In this study, a novel peptide grafted derivative of chitosan (CPP-g- chitosan) is synthesized and its potential for oral delivery of proteins and peptides is evaluated. Drug-loaded nanoparticles were developed from this derivative using ionic gelation method with application of sodium tripolyphosphate (TPP) as a cross-linking agent. Human insulin was used as the model protein drug and release kinetic was studied at gastrointestinal pH. Finally the developed nanoparticles were filled into very tiny enteric protective capsules and its effects on blood glucose level are evaluated in laboratory animals. Presence of the positively charged cell-penetrating peptide moiety in the structure of chitosan polymer had slight inhibitory effects on the release of insulin from the nanoparticles in simulated gastric fluid (pH 1.2) com...Continue Reading

Citations

Aug 25, 2019·Polymers·Shazia MansoorViness Pillay
Sep 3, 2020·Journal of Drug Targeting·Chun Y WongCrispin R Dass
Jun 2, 2020·Expert Opinion on Drug Delivery·Sandra RoblaNoemi S Csaba
Jun 30, 2019·Current Drug Delivery·Mohd H M Jaafar, Khuriah A Hamid
Mar 30, 2021·Acta Pharmaceutica Sinica. B·Ting ZhangQiang Peng

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Methods Mentioned

BETA
enzyme-linked immunosorbent assay
dynamic light scattering
ELISA

Software Mentioned

Agilent ChemStation
Advanced
Spectrum
Chemistry Developments lab

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