Synthesis and characterization of chimeric 2-5A-DNA oligonucleotides

Current Protocols in Nucleic Acid Chemistry
M R Player, P F Torrence

Abstract

This unit provides protocols for the synthesis and characterization of 2-5A-antisense nucleic acids. These chimeric oligonucleotides consist of 2',5'-phosphodiester-linked oligoadenylates ligated to 3',5'-deoxyribonucleotides and are readily prepared using phosphoramidite chemistry on CPG solid supports. The 3',5'-deoxyribonucleotide functions as the antisense domain to target a given mRNA sequence, while the 2',5'-phosphodiester-linked oligoadenylate serves to locally activate 2-5A-dependent RNase L, causing the targeted sequence to be cleaved.

References

Feb 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P F TorrenceR H Silverman
Jun 23, 1995·The Journal of Biological Chemistry·R K MaitraR H Silverman
Nov 1, 1993·Bioconjugate Chemistry·K LesiakP F Torrence
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·N M CirinoR H Silverman
Jun 12, 1998·Pharmacology & Therapeutics·M R Player, P F Torrence

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