Synthesis and characterization of Escherichia coli O18 O-polysaccharide conjugate vaccines.

Infection and Immunity
S J CryzE Fürer

Abstract

Nontoxic, serologically reactive O polysaccharide was derived from Escherichia coli O18 lipopolysaccharide by acid hydrolysis, extraction with organic solvents, and gel filtration chromatography. Oxidized O polysaccharide was covalently coupled to either Pseudomonas aeruginosa toxin A or cholera toxin by using adipic acid dihydrazide as a spacer molecule in the presence of carbodiimide. The resulting conjugates were composed of approximately equal amounts of O polysaccharide and protein and were nontoxic and nonpyrogenic. Both conjugates engendered an immunoglobulin G antibody response in rabbits that recognized native O18 lipopolysaccharide. Such antibody was able to promote the uptake and killing of an E. coli O18 strain bearing the K1 capsule by human polymorphonuclear leukocytes. Immunoglobulin G isolated from the sera of rabbits immunized with either conjugate afforded protection against an E. coli O18 challenge when passively transferred to mice.

References

Jul 11, 1987·Lancet·M AlpernJ Hooper
Oct 6, 1988·The New England Journal of Medicine·UNKNOWN Cooperative Group for the Study of Immunoglobulin in Chronic Lymphocytic LeukemiaDierdre Tait
Feb 1, 1984·The Journal of Infectious Diseases·A S CrossI Orskov

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Citations

Oct 23, 2001·Research in Veterinary Science·M C RebelattoH Hogenesch

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