Synthesis and characterization of insulin-like growth factor-binding protein (IGFBP)-7. Recombinant human mac25 protein specifically binds IGF-I and -II.

The Journal of Biological Chemistry
Y OhR G Rosenfeld

Abstract

The mac25 cDNA was originally cloned from leptomeningial cells and subsequently reisolated through differential display as a sequence preferentially expressed in senescent human mammary epithelial cells. The deduced amino acid sequence of the human mac25 propeptide shares a 20-25% identity to human insulin-like growth factor-binding proteins (IGFBPs), suggesting that mac25 could be another member of the IGFBP family. In the present study, we have generated recombinant human mac25 (rh-mac25) in a baculovirus expression system and assessed its affinity for IGFs and have evaluated the pattern of expression of the mac25 gene in human tissues. Binding of 125I-IGF-I and 125I-IGF-II to rh-mac25 was demonstrated by Western ligand blotting after nondenaturing polyacrylamide gel electrophoresis and by affinity cross-linking with as little as 2 nM rh-mac25. Specificity of rh-mac25 binding to 125I-IGFs was demonstrated by competition for rh-mac25 binding with unlabeled IGFs, but not with [QAYLL]IGF-II analog, which has 100-fold less affinity for IGFBPs. In comparison with IGFBP-3, rh-mac25 has at least a 5-6-fold lower affinity for IGF-I and 20-25-fold lower affinity for IGF-II. mac25 mRNA was detectable in a wide range of normal human tis...Continue Reading

References

Aug 1, 1991·The Journal of Clinical Endocrinology and Metabolism·P CohenR G Rosenfeld
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R C Baxter, J L Martin
Apr 1, 1994·Acta Paediatrica. Supplement·R G RosenfeldY Oh
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·K SwisshelmR Sager
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·S R NagallaE R Spindel
Feb 1, 1995·Endocrine Reviews·J I Jones, D R Clemmons

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Citations

Jun 17, 1998·International Journal of Cancer. Journal International Du Cancer·R TorrisiA Decensi
Aug 26, 1998·International Journal of Cancer. Journal International Du Cancer·M MenounyS Babajko
Jan 15, 2005·International Journal of Colorectal Disease·Rajaraman DuraiMarc C Winslet
Oct 3, 2003·Biochemical and Biophysical Research Communications·Sanjida AhmedKaoru Miyazaki
Aug 29, 1998·Molecular and Cellular Endocrinology·M van KleffensS L Drop
Aug 15, 1998·Molecular and Cellular Endocrinology·C Collet, J Candy
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M E WhiteW R Dayton
Feb 11, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E M Plisetskaya
Aug 7, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Mark R CorkinsRichard G MacDonald
Sep 15, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·H L SimpsonD L Russell-Jones
Jan 27, 2000·Cytokine & Growth Factor Reviews·B R LackeyD M Henricks
Mar 27, 1998·Breast Cancer Research and Treatment·Y Oh
Aug 10, 2000·Breast Cancer Research and Treatment·X L BourhisH Hondermarck
Feb 26, 2005·Nucleic Acids Research·Erez Y LevanonEli Eisenberg
Jan 22, 2005·International Journal of Urology : Official Journal of the Japanese Urological Association·Catherine CheungDavid Johnson
Dec 24, 1997·Acta Paediatrica. Supplement·R G Rosenfeld
Apr 2, 1999·Acta Paediatrica. Supplement·V HwaR G Rosenfeld
Dec 20, 2012·Science Signaling·Valentina EvdokimovaArun Seth
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Takayuki MatsumotoJeffrey B Kopp
Nov 21, 2012·Comparative Hepatology·Ingvild E NygårdArthur Revhaug
Jan 12, 2005·Reproductive Biology and Endocrinology : RB&E·Ujjwal K RoutMichael P Diamond

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