PMID: 9556758Apr 29, 1998Paper

Synthesis and characterization of polymer-(multi)-peptide conjugates for control of specific cell aggregation

Journal of Biomaterials Science. Polymer Edition
N BelchevaW M Saltzman

Abstract

A new synthetic approach has been applied to obtain novel di-, tetra-, and (multi)-peptide containing polymer conjugates in quantitative yields with a high degree of conjugation. Bis-(N-hydroxysuccinimidyl) esters of PEG (Mw = 200, 600, 1400, 2000, and 3400) were synthesized and studied in a condensation reaction with synthetic peptides: glycine-glycine-tyrosine-arginine (GGYR), a model peptide, and glycine-arginine-glycine-aspartic acid-tyrosine (GRGDY), a sequence known to promote cell adhesion and aggregation. Tetra-substituted derivatives of PEG-based conjugates were synthesized by coupling L-aspartic acid and L-aspartyl-L-phenylalanine through a condensation procedure in organic media. Poly(acrylic acid) and co-polymers (Mw = 2000 and 5000) were studied as a model of multifunctional linear polymers in the reaction with L-tryptophan and GGYR. Alternative polymer-(multi)-peptide conjugates were successfully synthesized using Starburst dendrimer PAMAM (G = 3), 'short' and 'long'-chain PEG-based active esters and GRGDY. The structure of the intermediate precursors and peptide-conjugates was confirmed by spectral (UV-Vis, FTIR, H-NMR) and chromatographic (RP-HPLC and SEC) methods. By varying the properties of the interconnectin...Continue Reading

References

Jan 1, 1995·Bioconjugate Chemistry·N Nakajima, Y Ikada
Jan 1, 1994·Virchows Archiv : an International Journal of Pathology·J PeringaW Timens
Mar 25, 1994·Science·N A Peppas, R Langer
May 1, 1993·Japanese Journal of Cancer Research : Gann·I SaikiI Azuma
May 20, 1996·Biotechnology and Bioengineering·W Dai, W M Saltzman

❮ Previous
Next ❯

Citations

Aug 19, 2003·Biomaterials·Ulrich HerselHorst Kessler
Jan 9, 2001·Trends in Biotechnology·J Gariépy, K Kawamura
Jun 6, 2000·Advanced Drug Delivery Reviews· Mark Saltzman W, S P Baldwin
Jun 19, 2002·Journal of Biotechnology·N TirelliJ A Hubbell
Nov 7, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Julia S ApteDavid G Castner
Apr 17, 2001·Tissue Engineering·S P Baldwin, W M Saltzman
Jun 26, 2002·Annals of the New York Academy of Sciences·Linda G Griffith
Apr 24, 2003·Annual Review of Physical Chemistry·G P DrobnyP S Stayton
Jul 7, 2007·The AAPS Journal·Tarek M FahmyW Mark Saltzman
Mar 24, 2007·Nanomedicine : Nanotechnology, Biology, and Medicine·Tarek M FahmyW Mark Saltzman
Apr 20, 2010·Journal of Biomedical Materials Research. Part a·Rebecca ScottRebecca Kuntz Willits
Nov 5, 2005·Journal of Biomedical Materials Research. Part a·Hu Yang, Stephanie T Lopina
May 30, 2002·Journal of the American Chemical Society·Joanna R LongPatrick S Stayton
Mar 23, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·P V BowerG P Drobny
Feb 19, 2002·Chemical Reviews·A. W. BosmanE. W. Meijer

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.