Synthesis and characterization of silica-based hyper-crosslinked sulfonate-modified reversed stationary phases.

Journal of Chromatography. a
Hao LuoPeter W Carr

Abstract

A novel type of silica-based sulfonate-modified reversed phase ((-)SO3-HC-C8) has been synthesized; it is based on a newly developed acid stable "hyper-crosslinked" C8 derivatized reversed phase, denoted HC-C8. The (-)SO3-HC-C8 phases containing controlled amounts of sulfonyl groups were made by sulfonating the aromatic hyper-crosslinked network of the HC-C(8) phase at different temperatures. The (-)SO3-HC-C8 phases are only slightly less hydrophobic than the parent HC-C8 phase. The added sulfonyl groups provide a unique strong cation-exchange selectivity to the hydrophobic hyper-crosslinked substrate as indicated by the very large C coefficient as shown through Snyder's hydrophobic subtraction reversed-phase characterization method. This cation-exchange activity clearly distinguishes the sulfonated phase from all other reversed phases as confirmed by the very high values of Snyder's column comparison function F(s). In addition, as was found in previous studies of silica-based and zirconia-based reversed phases, a strong correlation between the cation-exchange interaction and hydrophobic interaction was observed for these sulfonated phases in studies of the retention of cationic solutes. The overall chromatographic selectivity ...Continue Reading

References

Jul 13, 2000·Journal of Chromatography. a·J LiJ S Fritz
Aug 28, 2003·Journal of the American Chemical Society·Brian C TrammellPeter W Carr
Sep 4, 2004·Journal of Biochemical and Biophysical Methods·Tatsunari Yoshida
Sep 14, 2004·Journal of Proteome Research·Kiyonaga FujiiToshihide Nishimura
Jan 5, 2005·Journal of Chromatography. a·H A Claessens, M A van Straten
Jan 25, 2005·The Analyst·Brett Paull, Pavel N Nesterenko
Feb 1, 2005·Journal of Chromatography. a·D H MarchandL R Snyder
Aug 8, 2006·Journal of Chromatography. a·Mark Vitha, Peter W Carr

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Citations

Feb 16, 2008·Analytical and Bioanalytical Chemistry·H Luo, P W Carr
Jun 29, 2010·Analytical Chemistry·Melanie DumareySarah C Rutan

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