Synthesis and evaluation of a glutamic acid-modified hPAMAM complex as a promising versatile gene carrier

Journal of Drug Targeting
Mohammad HemmatiReza Shirkoohi

Abstract

Hyperbranched poly(amidoamine) (HPAMAM), structurally analogous to polyamidoamine dendrimer (PAMAM) dendrimers, has been suggested to be an effective carrier for gene delivery. In the present study, glutamic acid-modified hPAMAM was developed as a novel non-viral gene carrier for the first time. The hPAMAM was synthesized by using a modified one-pot method. DNA was found to be bound to hPAMAM at different weight ratios (WhPAMAM/WDNA). The resulting HPAMAM-Glu20 was able to efficiently protect the encapsulated-DNA against degradation for over 2 h. In addition to low cytotoxicity, the transfection efficiency of hPAMAM-Glu20 represented much higher (p < 0.05) than that of Lipofectamine 2000 in both MCF7 and MDA-MB231 cells. Cellular uptake of the hPAMAM-Glu20 in MDA-MB231 cells, 173.56 ± 1.37%, was significantly higher than that of MCF7 cells, 65.00 ± 1.73% (p < 0.05). The results indicated that hPAMAM-Glu20-mediated gene delivery to breast cancer cells is a feasible and effective strategy that may provide a new therapeutic avenue as a non-viral gene delivery carrier. In addition, it was found that hPAMAM-glutamic amino acid (Glu)-based gene delivery is an economical, effective and biocompatible method.

References

May 14, 1996·Proceedings of the National Academy of Sciences of the United States of America·J F Kukowska-LatalloJ R Baker
Nov 1, 1996·Bioconjugate Chemistry·M X TangF C Szoka
Sep 30, 1999·Bioconjugate Chemistry·A U BielinskaJ R Baker
Apr 27, 2001·Biochemical and Biophysical Research Communications·B J RoesslerJ R Baker
Apr 12, 2002·Proceedings of the National Academy of Sciences of the United States of America·Donald A TomaliaDouglas R Swanson
Aug 16, 2002·Pharmaceutical Research·Bernd H ZinselmeyerIjeoma F Uchegbu
Nov 2, 2002·Nature Medicine·Laura Bonetta
May 3, 2003·Nature Reviews. Genetics·Clare E ThomasMark A Kay
Feb 18, 2005·NeuroRx : the Journal of the American Society for Experimental NeuroTherapeutics·William M Pardridge
Jul 20, 2005·The Journal of Gene Medicine·Salvatore CarrabinoMassimo Conese
Aug 28, 2007·The Journal of Gene Medicine·Michael L EdelsteinJo Wixon
Aug 12, 2008·Mini Reviews in Medicinal Chemistry·Yu GaoRong Qi
Apr 7, 2010·International Journal of Pharmaceutics·Ajay KumarSrinath Palakurthi
Feb 7, 2013·International Journal of Pharmaceutics·Cheng Zhe BaiJong-Sang Park
Jul 23, 2013·Nanomedicine : Nanotechnology, Biology, and Medicine·Samina NazirAlexander John MacRobert
Apr 24, 2015·Chemical Society Reviews·Yaochen ZhengChao Gao

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Citations

Mar 1, 2019·Turkish Journal of Biology = Türk Biyoloji Dergisi·Amin AmaniRasool Asghari-Zakaria
Nov 12, 2021·Macromolecular Rapid Communications·Meike N Leiske, Kristian Kempe

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