Synthesis and evaluation of dual crosslinked alginate microbeads

Acta Biomaterialia
Sami I SomoEric M Brey

Abstract

Alginate hydrogels have been investigated for a broad variety of medical applications. The ability to assemble hydrogels at neutral pH and mild temperatures makes alginate a popular choice for the encapsulation and delivery of cells and proteins. Alginate has been studied extensively for the delivery of islets as a treatment for type 1 diabetes. However, poor stability of the encapsulation systems after implantation remains a challenge. In this paper, alginate was modified with 2-aminoethyl methacrylate hydrochloride (AEMA) to introduce groups that can be photoactivated to generate covalent bonds. This enabled formation of dual crosslinked structure upon exposure to ultraviolet light following initial ionic crosslinking into bead structures. The degree of methacrylation was varied and in vitro stability, long term swelling, and cell viability examined. At low levels of the methacrylation, the beads could be formed by first ionic crosslinks followed by exposure to ultraviolet light to generate covalent bonds. The methacrylated alginate resulted in more stable beads and cells were viable following encapsulation. Alginate microbeads, ionic (unmodified) and dual crosslinked, were implanted into a rat omentum pouch model. Implantati...Continue Reading

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Citations

May 28, 2019·Annals of Biomedical Engineering·Jacob BrownEric Brey
Nov 27, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ionut-Cristian RaduBianca Galateanu
Jun 20, 2019·Frontiers in Bioengineering and Biotechnology·Shuixan Hu, Paul de Vos
Dec 8, 2020·Frontiers in Chemistry·Sami I SomoEric M Brey
Jan 15, 2021·Biomedical Physics & Engineering Express·Alexander Sturtivant, Anthony Callanan
Jun 12, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tania B Lopez-MendezRosa Maria Hernandez
Jul 3, 2021·Cancers·Dariusz LachowskiArmando E Del Río Hernández
Jan 8, 2020·ACS Applied Materials & Interfaces·Felipe Olate-MoyaHumberto Palza
Aug 28, 2021·International Journal of Molecular Sciences·Teruki Nii, Yoshiki Katayama
Nov 24, 2021·Topics in Current Chemistry·Chandan Maity, Nikita Das

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