PMID: 8612043Jan 1, 1995Paper

Synthesis and evaluation of oligodeoxynucleotides containing acyclic nucleosides: introduction of three novel analogues and a summary

Bioorganic & Medicinal Chemistry
P NielsenJ Wengel

Abstract

Novel flexible oligodeoxynucleotides containing (S)-1-(2,3-dihydroxypropyl)thymine or 2',3'-seco-thymidine nucleoside analogues were synthesized on an automated DNA-synthesizer. Oligodeoxynucleotides with one, two or three acyclic nucleosides incorporated in the middle or in the ends of 17-mers have been evaluated. 3'-End-modified oligomers were significantly stabilized towards 3'-exonucleolytic degradation compared to unmodified analogues and showed acceptable hybridization properties as measured by UV experiments. For oligodeoxynucleotide analogues containing the three novel acyclic monomers in the middle, a more pronounced reduction in duplex stability was observed. All oligodeoxynucleotides containing acyclic nucleoside analogues made so far are evaluated with respect to stability towards 3'-exonucleolytic degradation and hybridization properties.

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Citations

Jan 27, 2004·Bioorganic & Medicinal Chemistry Letters·Vyacheslav V Filichev, Erik B Pedersen
Mar 3, 2011·Molecular Therapy : the Journal of the American Society of Gene Therapy·Shaguna SethBarry Polisky
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