Synthesis and grafting of folate-PEG-PAMAM conjugates onto quantum dots for selective targeting of folate-receptor-positive tumor cells

Journal of Colloid and Interface Science
Yili ZhaoJingyuan Wang

Abstract

We report the design and synthesis of folate-poly(ethylene glycol)-polyamidoamine (FPP)-functionalized CdSe/ZnS quantum dots (QDs), in which the QD plays a key role in imaging, whereas the folate-poly(ethylene glycol) (PEG) conjugates of the polyamidoamine (PAMAM) dendrimer serve as a system targeted to folate receptors in tumor cells. Dendrimer ligands such as folate-PEG grafted PAMAM of generation 3.5 are found to encapsulate and solubilize luminescent QDs through direct ligand-exchange reactions. Because of membrane expression of FA receptors in tumor cells, this class of ligand-exchanged QDs is able to target tumor cells. We have evaluated FPP-coated QDs and QDs without folate in HeLa cells and shown that cellular uptake of FPP-coated QDs is more significant than that non folate QDs in vivo imaging experiment. In particular, QDs coated with FPP are initially bound to tumor cell surfaces, followed by slow endosomal escape and release into the tumor cells. These insights are important for the design and development of nanoparticle agents for optical detection of tumor cells and bio-imaging.

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Citations

Jun 15, 2013·Pharmaceutical Research·Zhe WangXiaoyuan Chen
Dec 3, 2011·Expert Review of Molecular Diagnostics·Jörn ProbstSabine Rupp
Mar 2, 2016·Nature Nanotechnology·Eunkeu OhIgor L Medintz
Aug 19, 2016·Journal of Biomaterials Science. Polymer Edition·Mustafa U GürbüzMetin Tülü
Dec 3, 2016·Chemical Biology & Drug Design·Manli SongXianzhong Zhang
Jun 7, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Ramesh NandiSamir Kumar Pal
Sep 14, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jingyi Zhu, Xiangyang Shi
Mar 28, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Bing XueYue-Qing Gu
Jul 8, 2015·Nanotechnology·Ying-Ying MaHuan-Ming Xiong
Nov 26, 2019·Materials Science & Engineering. C, Materials for Biological Applications·Batoul FarranGanji Seeta Rama Raju
Mar 7, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Serge MignaniJean-Pierre Majoral
Feb 23, 2021·Asian Journal of Pharmaceutical Sciences·Panoraia I SiafakaEvren Atlıhan Gündoğdu

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