Synthesis and immunological properties of Vi and di-O-acetyl pectin protein conjugates with adipic acid dihydrazide as the linker.

Infection and Immunity
Z KossaczkaS C Szu

Abstract

The Vi capsular polysaccharide of Salmonella typhi, a licensed vaccine for typhoid fever in individuals > or = 5 years old, induces low and short-lived antibodies in children, and reinjection does not elicit booster responses at any age. Its immunogenicity was improved by binding Vi to proteins by using N-succinimidyl-3-(2-pyridyldithio)propionate (SPDP) as a linker. Similar findings were observed with the structurally related, di-O-acetyl derivative of pectin [poly-alpha(1-->4)-D-GalpA] designated OAcP. Protein conjugates of Vi and OAcP were synthesized by carbodiimide-mediated synthesis with adipic acid dihydrazide (ADH) as the linker. Hydrazide groups were introduced into proteins (bovine serum albumin or recombinant Pseudomonas aeruginosa exoprotein A) by treatment with ADH and 1-ethyl-3(3-dimethylaminopropyl carbodiimide (EDC). The resultant adipic acid hydrazide derivatives (AH-proteins), containing 2.3 to 3.4% AH, had antigenic and physicochemical properties similar to those of the native proteins. The AH-proteins were bound to Vi and OAcP by treatment with EDC. The immunogenicity of Vi or OAcP, alone or as protein conjugates, was evaluated in young outbred mice and guinea pigs by subcutaneous injection of 2.5 and 5.0 mi...Continue Reading

References

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Citations

Apr 26, 2001·The New England Journal of Medicine·F Y LinS C Szu
Feb 19, 2016·Expert Review of Vaccines·Sergio Rosales-MendozaRalf Reski
Mar 7, 2020·The Indian Journal of Medical Research·Vipin M Vashishtha, Ajay Kalra
Dec 23, 2003·Infection and Immunity·Margret Y RichterIngileif Jonsdottir
May 17, 2005·Anais Da Academia Brasileira De Ciências·Christopher Jones
Oct 26, 2013·Expert Review of Vaccines·Shousun Chen Szu
Jan 24, 2018·Human Vaccines & Immunotherapeutics·Erman TritamaDebbie Sofie Retnoningrum
Oct 14, 2019·Biologicals : Journal of the International Association of Biological Standardization·Shabirul HaqueBansilal Jailkhani
May 8, 2000·Biologicals : Journal of the International Association of Biological Standardization·X LemercinierC Jones
Mar 30, 2002·Molecular Immunology·Fernando J IrazoquiGustavo A Nores
Jul 13, 2021·Biologicals : Journal of the International Association of Biological Standardization·P V S L S S Narayana, Jayati Ray Dutta
Jul 22, 2006·Carbohydrate Research·David Scott SnyderParastoo Azadi

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