Synthesis and in vitro evaluation of thermosensitive hydrogel scaffolds based on (PNIPAAm-PCL-PEG-PCL-PNIPAAm)/Gelatin and (PCL-PEG-PCL)/Gelatin for use in cartilage tissue engineering

Journal of Biomaterials Science. Polymer Edition
Solmaz SaghebaslAli Ramazani

Abstract

Biodegradable thermosensitive hydrogel scaffolds based on novel three-block PCL-PEG-PCL and penta block PNIPAAm-PCL-PEG-PCL-PNIPAAm copolymers blended with gelatin were prepared and examined on functional behavior of chondrocytes. In this work, we compared two different thermosensitive hydrogel scaffolds (PNIPAAm-PCL-PEG-PCL-PNIPAAm)/Gelatin and (PCL-PEG-PCL)/Gelatin prepared by TIPS (thermally induced phase separation) method. The feature of copolymers was characterized by FT-IR, 1H NMR. The lower critical solution temperatures (LCSTs) of aqueous solutions of copolymers were measured by cloud point (turbidity) measurements. We also examined water absorption capacity and swelling ratio. Mechanical features of the prepared hydrogels were evaluated by stress-strain measurements. Thereafter, isolated chondrocytes were cultured on each scaffold for a period of 10 days and cell arrangement and morphology studied pre-and post-plating. Cell survival assay was done by using MTT assay. The transcription level of genes Sox-9, Collagen-II, COMP, MMP-13 and oligomeric matrix protein was monitored by real-time PCR assay. The samples were also stained by Toluidine blue method to monitor the synthesis of proteoglycan. Data demonstrated an inc...Continue Reading

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Citations

May 16, 2018·Molecular Biotechnology·Nadeem SiddiquiSreenivasa Rao
Jan 17, 2019·Tissue Engineering. Part B, Reviews·Mohsen SetayeshmehrMohammad T Joghataei
Aug 15, 2019·Artificial Cells, Nanomedicine, and Biotechnology·Mahdieh AlipourVahideh Raeisdasteh Hokmabad
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Cornelia VasileMihaela Baican
Apr 6, 2020·Biotechnology and Applied Biochemistry·Hessam RezaeiMohammadali Sahebalzamani
Jul 13, 2019·Current Medicinal Chemistry·Yasamin Davatgaran TaghipourHamid Tayefi Nasrabadi
Oct 27, 2020·Bioactive Materials·Wei WeiHongwei Ouyang
Apr 13, 2021·Frontiers in Bioengineering and Biotechnology·Xia ZhaoAravind Athiviraham
Mar 8, 2019·Biomacromolecules·Yanbo ZhangXuesi Chen
Nov 24, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Tongmeng JiangChuanbin Mao

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Methods Mentioned

BETA
chemical modification
nuclear magnetic resonance
PCEC
ELISA
electrophoresis
PCR

Software Mentioned

image J
Cell sense

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